Disorders on cardiovascular parameters in rats and in human blood cells caused by Lachesis acrochorda snake venom.

Autor: Angel-Camilo KL; Departamento de Fisiologia e Farmacologia, Faculdade de Medicina, Universidade Federal Do Ceará, Fortaleza, Ceará, Brazil; Facultad de Ciencias Naturales, Exactas y de la Educación, Departamento de Biología, Centro de Investigaciones Biomédicas-Bioterio, Grupo de Investigaciones Herpetológicas y Toxinológicas, Universidad del Cauca, Popayán, Colombia., Guerrero-Vargas JA; Facultad de Ciencias Naturales, Exactas y de la Educación, Departamento de Biología, Centro de Investigaciones Biomédicas-Bioterio, Grupo de Investigaciones Herpetológicas y Toxinológicas, Universidad del Cauca, Popayán, Colombia., Carvalho EF; Departamento de Fisiologia e Farmacologia, Faculdade de Medicina, Universidade Federal Do Ceará, Fortaleza, Ceará, Brazil., Lima-Silva K; Departamento de Fisiologia e Farmacologia, Faculdade de Medicina, Universidade Federal Do Ceará, Fortaleza, Ceará, Brazil., de Siqueira RJB; Departamento de Fisiologia e Farmacologia, Faculdade de Medicina, Universidade Federal Do Ceará, Fortaleza, Ceará, Brazil., Freitas LBN; Centro de Estudos Farmacêuticos e Cosméticos, Faculdade de Farmácia, Odontologia e Enfermagem, Universidade Federal Do Ceará, Fortaleza, Ceará, Brazil., Sousa JAC; Centro de Estudos Farmacêuticos e Cosméticos, Faculdade de Farmácia, Odontologia e Enfermagem, Universidade Federal Do Ceará, Fortaleza, Ceará, Brazil., Mota MRL; Departamento de Fisiologia e Farmacologia, Faculdade de Medicina, Universidade Federal Do Ceará, Fortaleza, Ceará, Brazil., Santos AAD; Departamento de Fisiologia e Farmacologia, Faculdade de Medicina, Universidade Federal Do Ceará, Fortaleza, Ceará, Brazil., Neves-Ferreira AGDC; Laboratório de Toxinologia, Instituto Oswaldo Cruz, Fiocruz, Fiocruz, Av. Brasil 4365, Manguinhos, Rio de Janeiro, 21040-900, Brazil., Havt A; Departamento de Fisiologia e Farmacologia, Faculdade de Medicina, Universidade Federal Do Ceará, Fortaleza, Ceará, Brazil., Leal LKAM; Centro de Estudos Farmacêuticos e Cosméticos, Faculdade de Farmácia, Odontologia e Enfermagem, Universidade Federal Do Ceará, Fortaleza, Ceará, Brazil., Magalhães PJC; Departamento de Fisiologia e Farmacologia, Faculdade de Medicina, Universidade Federal Do Ceará, Fortaleza, Ceará, Brazil. Electronic address: pjcmagal@ufc.br.
Jazyk: angličtina
Zdroj: Toxicon : official journal of the International Society on Toxinology [Toxicon] 2020 Sep; Vol. 184, pp. 180-191. Date of Electronic Publication: 2020 Jun 23.
DOI: 10.1016/j.toxicon.2020.06.009
Abstrakt: In Colombia, Lachesis acrochorda causes 2-3% of all snake envenomations. The accidents promote a high mortality rate (90%) due to blood and cardiovascular complications. Here, the effects of the snake venom of L. acrochorda (SVLa) were analyzed on human blood cells and on cardiovascular parameters of rats. SVLa induced blood coagulation, as measured by the prothrombin time test, but did not reduce the cell viability of neutrophils and platelets evaluated by the 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyl tetrazolium bromide (MTT) reduction assay and by the lactate dehydrogenase (LDH) enzyme assay. In fact, SVLa increased the absorbance in tests made with platelets subjected to the MTT assay. SVLa induced platelet aggregation whose magnitude was comparable to that of the positive control adenosine diphosphate (ADP), and occurred earlier with increasing SVLa concentration. Acetylsalicylic acid (ASA, a cyclooxygenase inhibitor) or clopidogrel (an ADP receptor blocker) inhibited the aggregating effect of SVLa. Inhibition of SVLa-elicited platelet aggregation also resulted from the treatment with disodium ethylenediaminetetraacetate (Na 2 -EDTA; metalloproteinase inhibitor) and with 4-(2-aminoethyl) benzenesulfonyl fluoride hydrochloride (AEBSF, serine protease inhibitor). In isolated right atrium of rats, SVLa increased slightly, but significantly, the magnitude of the spontaneous contractions and, in isolated rat aorta, SVLa relaxed KCl- or phenylephrine-induced contractions. In vivo, SVLa induced hypotension and bradycardia in rats, with detection of hemorrhage in pulmonary and renal tissues. Altogether, under experimental conditions, SVLa induced blood coagulation, platelet aggregation, hypotension and bradycardia. Part of the effects presented here may be explained by the presence of snake venom metalloproteinases (SVMPs) and snake venom serine proteases (SVSPs), constituents of SVLa.
(Copyright © 2020 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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