Specific labeling of synaptic schwann cells reveals unique cellular and molecular features.

Autor:	Castro R; Department of Molecular Biology, Cellular Biology, and Biochemistry, Brown University, Providence, United States.; Center for Translational Neuroscience, Robert J. and Nancy D. Carney Institute for Brain Science and Brown Institute for Translational Science, Brown University, Providence, United States.; Neuroscience Graduate Program, Brown University, Providence, United States., Taetzsch T; Department of Molecular Biology, Cellular Biology, and Biochemistry, Brown University, Providence, United States.; Center for Translational Neuroscience, Robert J. and Nancy D. Carney Institute for Brain Science and Brown Institute for Translational Science, Brown University, Providence, United States., Vaughan SK; Department of Molecular Biology, Cellular Biology, and Biochemistry, Brown University, Providence, United States.; Center for Translational Neuroscience, Robert J. and Nancy D. Carney Institute for Brain Science and Brown Institute for Translational Science, Brown University, Providence, United States., Godbe K; Fralin Biomedical Research Institute at Virginia Tech Carilion, Roanoke, United States., Chappell J; Fralin Biomedical Research Institute at Virginia Tech Carilion, Roanoke, United States., Settlage RE; Department of Advanced Research Computing, Virginia Tech, Blacksburg, United States., Valdez G; Department of Molecular Biology, Cellular Biology, and Biochemistry, Brown University, Providence, United States.; Center for Translational Neuroscience, Robert J. and Nancy D. Carney Institute for Brain Science and Brown Institute for Translational Science, Brown University, Providence, United States.; Department of Neurology, Warren Alpert Medical School of Brown University, Providence, United States.
Jazyk:	angličtina
Zdroj:	ELife [Elife] 2020 Jun 25; Vol. 9. Date of Electronic Publication: 2020 Jun 25.
DOI:	10.7554/eLife.56935
Abstrakt:	Perisynaptic Schwann cells (PSCs) are specialized, non-myelinating, synaptic glia of the neuromuscular junction (NMJ), that participate in synapse development, function, maintenance, and repair. The study of PSCs has relied on an anatomy-based approach, as the identities of cell-specific PSC molecular markers have remained elusive. This limited approach has precluded our ability to isolate and genetically manipulate PSCs in a cell specific manner. We have identified neuron-glia antigen 2 (NG2) as a unique molecular marker of S100β+ PSCs in skeletal muscle. NG2 is expressed in Schwann cells already associated with the NMJ, indicating that it is a marker of differentiated PSCs. Using a newly generated transgenic mouse in which PSCs are specifically labeled, we show that PSCs have a unique molecular signature that includes genes known to play critical roles in PSCs and synapses. These findings will serve as a springboard for revealing drivers of PSC differentiation and function. Competing Interests: RC, TT, SV, KG, JC, RS, GV No competing interests declared (© 2020, Castro et al.)
Databáze:	MEDLINE
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