One Clonal Lineage of Calonectria pseudonaviculata Is Primarily Responsible for the Boxwood Blight Epidemic in the United States.

Autor: Castroagudín VL; U.S. Department of Agriculture-Agricultural Research Service, Mycology and Nematology Genetic Diversity and Biology Laboratory, Beltsville, MD 20705.; Oak Ridge Institute for Science and Education, ARS Research Participation Program, Oak Ridge, TN 37830., Weiland JE; U.S. Department of Agriculture-Agricultural Research Service, Horticultural Crops Research Laboratory, Corvallis, OR 97339., Baysal-Gurel F; Department of Agricultural and Environmental Sciences, Otis L. Floyd Nursery Research Center, Tennessee State University, McMinnville, TN 37110., Cubeta MA; Center for Integrated Fungal Research, Department of Entomology and Plant Pathology, North Carolina State University, Raleigh, NC 27606., Daughtrey ML; School of Integrative Plant Science, College of Agriculture and Life Sciences, Cornell University, Ithaca, NY 14853., Gauthier NW; Department of Plant Pathology, University of Kentucky, Lexington, KY 40546., LaMondia J; Connecticut Agricultural Experiment Station, Valley Laboratory, Windsor, CT 06095., Luster DG; U.S. Department of Agriculture-Agricultural Research Service, Foreign Disease-Weed Science Research Unit, Frederick, MD 21702., Hand FP; Department of Plant Pathology, The Ohio State University, Columbus, OH 43210., Shishkoff N; U.S. Department of Agriculture-Agricultural Research Service, Foreign Disease-Weed Science Research Unit, Frederick, MD 21702., Williams-Woodward J; Department of Plant Pathology, University of Georgia, Athens, GA 30602., Yang X; Oak Ridge Institute for Science and Education, ARS Research Participation Program, Oak Ridge, TN 37830.; U.S. Department of Agriculture-Agricultural Research Service, Foreign Disease-Weed Science Research Unit, Frederick, MD 21702., LeBlanc N; U.S. Department of Agriculture-Agricultural Research Service, Mycology and Nematology Genetic Diversity and Biology Laboratory, Beltsville, MD 20705.; Oak Ridge Institute for Science and Education, ARS Research Participation Program, Oak Ridge, TN 37830.; Center for Integrated Fungal Research, Department of Entomology and Plant Pathology, North Carolina State University, Raleigh, NC 27606., Crouch JA; U.S. Department of Agriculture-Agricultural Research Service, Mycology and Nematology Genetic Diversity and Biology Laboratory, Beltsville, MD 20705.
Jazyk: angličtina
Zdroj: Phytopathology [Phytopathology] 2020 Nov; Vol. 110 (11), pp. 1845-1853. Date of Electronic Publication: 2020 Oct 07.
DOI: 10.1094/PHYTO-04-20-0130-R
Abstrakt: Boxwood blight caused by Calonectria pseudonaviculata and C. henricotiae is destroying cultivated and native boxwood worldwide, with profound negative economic impacts on the horticulture industry. First documented in the United States in 2011, the disease has now occurred in 30 states. Previous research showed that global C. pseudonaviculata populations prior to 2014 had a clonal structure, and only the MAT1-2 idiomorph was observed. In this study, we examined C. pseudonaviculata genetic diversity and population structure in the United States after 2014, following the expansion of the disease across the country over the past 5 years. Two hundred eighteen isolates from 21 states were genotyped by sequencing 11 simple sequence repeat (SSR) loci and by MAT1 idiomorph typing. All isolates presented C. pseudonaviculata -specific alleles, indicating that C. henricotiae is still absent in the U.S. states sampled. The presence of only the MAT1-2 idiomorph and gametic linkage disequilibrium suggests the prevalence of asexual reproduction. The contemporary C. pseudonaviculata population is characterized by a clonal structure and composed of 13 multilocus genotypes (SSR-MLGs) unevenly distributed across the United States. These SSR-MLGs grouped into two clonal lineages (CLs). The predominant lineage CL2 (93% of isolates) is the primary contributor to U.S. disease expansion. The contemporary U.S. C. pseudonaviculata population is not geographically subdivided and not genetically differentiated from the U.S. population prior to 2014, but is significantly differentiated from the main European population, which is largely composed of CL1. Our findings provide insights into the boxwood blight epidemic that are critical for disease management and breeding of resistant boxwood cultivars.
Databáze: MEDLINE
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