Assessing the Contributions of Inactivation, Removal, and Transfer of Ebola Virus and Vesicular Stomatitis Virus by Disinfectant Pre-soaked Wipes.

Autor: Cutts TA; Canadian Science Centre for Human and Animal Health, Winnipeg, MB, Canada.; J.C. Wilt Infectious Diseases Research Centre, Public Health Agency of Canada, Winnipeg, MB, Canada., Robertson C; Canadian Science Centre for Human and Animal Health, Winnipeg, MB, Canada.; J.C. Wilt Infectious Diseases Research Centre, Public Health Agency of Canada, Winnipeg, MB, Canada., Theriault SS; Department of Microbiology, The University of Manitoba, Winnipeg, MB, Canada., Nims RW; RMC Pharmaceutical Solutions, Inc., Longmont, CO, United States., Kasloff SB; Canadian Science Centre for Human and Animal Health, Winnipeg, MB, Canada.; J.C. Wilt Infectious Diseases Research Centre, Public Health Agency of Canada, Winnipeg, MB, Canada., Rubino JR; Reckitt Benckiser LLC, Global Research & Development for Lysol and Dettol, Montvale, NJ, United States., Ijaz MK; Reckitt Benckiser LLC, Global Research & Development for Lysol and Dettol, Montvale, NJ, United States.; Department of Biology, Medgar Evers College of the City University of New York (CUNY), Brooklyn, NY, United States.
Jazyk: angličtina
Zdroj: Frontiers in public health [Front Public Health] 2020 Jun 02; Vol. 8, pp. 183. Date of Electronic Publication: 2020 Jun 02 (Print Publication: 2020).
DOI: 10.3389/fpubh.2020.00183
Abstrakt: Disinfectant pre-soaked wipes (DPW) containing activated hydrogen peroxide (AHP) or quaternary ammonium compounds (QAC) were tested using ASTM E2967-15 to determine removal, transfer, and inactivation of Ebola virus Makona variant (EBOV/Mak) and vesicular stomatitis virus (VSV) from contaminated stainless steel prototypic environmental surfaces. The infectious virus-contaminated carriers were subjected to wiping in the Wiperator per the standard. Following the use of negative control (J-Cloth)-, AHP-, or QAC-based wipes, recovery of residual infectious virus was assayed. In the case of the J-Cloth wipes (negative control), although removal of virus from inoculated carriers was extensive i.e., ~99% (1.9-3.5 log 10 ) transfer of virus by these wipes to a secondary surface amounted to ≤ 2% (~3.8 log 10 ) of the initial virus load. In the case of each DPW, >6 log 10 removal/inactivation of virus was observed, with limited (EBOV/Mak) or no (VSV) virus transfer observed. The efficacy of wipes for decontaminating high-touch environmental surfaces spiked with EBOV/Mak or VSV is discussed. In summary, removal of EBOV/Mak and VSV using wipes was extensive in this study. In the absence of a sufficient concentration and contact time of an appropriate microbicidal active in DPW (such as the AHP- and QAC-based DPW tested), transfer of a low, albeit significant (from an infectious unit/infectious dose perspective), quantity of infectious virus from the inoculated surface to a secondary surface was observed. In the case of Ebola virus, it is essential that a DPW with an appropriate microbicidal active, following the appropriate contact time, be used to prevent unintended transfer of infectious virus to a clean secondary surface (as observed in negative control /J-Cloth). Otherwise, there exists the possibility of dissemination of Ebola virus and the associated risk of transmission of Ebola virus disease.
(Copyright © 2020 Cutts, Robertson, Theriault, Nims, Kasloff, Rubino and Ijaz.)
Databáze: MEDLINE