The Naphthalene Catabolic Genes of Pseudomonas putida BS3701: Additional Regulatory Control.

Autor:	Pozdnyakova-Filatova I; Laboratory of Molecular Microbiology, Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences, Federal Research Center, G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Pushchino, Russia., Petrikov K; Laboratory of Plasmid Biology, Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences, Federal Research Center, G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Pushchino, Russia., Vetrova A; Laboratory of Plasmid Biology, Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences, Federal Research Center, G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Pushchino, Russia., Frolova A; Laboratory of Bacteriophage Biology, Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences, Federal Research Center, G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Pushchino, Russia., Streletskii R; Laboratory of Ecological Soil Science, Lomonosov Moscow State University, Moscow, Russia., Zakharova M; Laboratory of Molecular Microbiology, Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences, Federal Research Center, G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Pushchino, Russia.
Jazyk:	angličtina
Zdroj:	Frontiers in microbiology [Front Microbiol] 2020 Jun 05; Vol. 11, pp. 1217. Date of Electronic Publication: 2020 Jun 05 (Print Publication: 2020).
DOI:	10.3389/fmicb.2020.01217
Abstrakt:	Pseudomonas microorganisms are used for bioremediation of soils contaminated with petroleum hydrocarbons. The overall remediation efficiency is largely dependent on the presence of macro- and micronutrients. Widely varying concentrations of available nitrogen and iron (Fe) in soils were shown to affect residual hydrocarbons in the course of biodegradation. The regulatory mechanisms of expression of hydrocarbon catabolic genes in low nitrogen/low iron conditions remain unclear. The catabolism of naphthalene, a two-ring polycyclic aromatic hydrocarbon, has been well studied in pseudomonads in terms of the involvement of specific transcriptional activators, thus making it useful in revealing additional regulatory control of the adaptation of hydrocarbon destructors to a low level of the essential nutrients. The Pseudomonas putida strain BS3701 is a component of the "MicroBak" preparation for soil remediation. Previously, this strain was shown to contain genes encoding the key enzymes for naphthalene catabolism: naphthalene 1,2-dioxygenase, salicylate hydroxylase, catechol 2,3-dioxygenase, and catechol 1,2-dioxygenase. Our study aimed to clarify whether the naphthalene catabolic gene expression is dependent on the amount of nitrogen and iron in the growth culture medium, and if so, at exactly which stages the expression is regulated. We cultivated the strain in low nitrogen/low iron conditions with the concurrent evaluation of the activity of the key enzymes and the mRNA level of genes encoding these enzymes. We are the first to report that naphthalene catabolic genes are subject not only to transcriptional but also post-transcriptional regulation. (Copyright © 2020 Pozdnyakova-Filatova, Petrikov, Vetrova, Frolova, Streletskii and Zakharova.)
Databáze:	MEDLINE
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