| Autor: |
Buckman TD; Department of Psychiatry and Biobehavioral Sciences Medical School, University of California, Los Angeles 90024., Chang R, Sutphin MS, Eiduson S |
| Jazyk: |
angličtina |
| Zdroj: |
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 1988 Mar 15; Vol. 151 (2), pp. 897-904. |
| DOI: |
10.1016/s0006-291x(88)80366-8 |
| Abstrakt: |
When uptake of the Parkinson's syndrome inducing neurotoxin MPTP (1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine) and its major brain metabolite MPP+ (1-methyl-4-phenylpyridinium ion) by human platelets were compared in platelet rich plasma, a much higher rate was observed for the metabolite. The uptake process was saturable (Km = 6.8 microM; Vmax = 0.064 nmole/min/mg protein) and could be blocked by inhibitors of serotonin uptake. The accumulation of MPP+ by the platelets was accompanied by a decrease in intracellular ATP and an inhibition of mitochondrial state 3 respiration. These findings are consistent with earlier reports of the effect of MPP+ on isolated mitochondria as a potential cytotoxic mechanism, but also demonstrate that the dopamine uptake system is not the only means by which this metabolite can be efficiently transported into cells. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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