Extracellular Release of Antigen by Dendritic Cell Regurgitation Promotes B Cell Activation through NF-κB/cRel.

Autor:	El-Barbry H; Université de Paris, Institut Cochin, INSERM, U1016, CNRS, UMR 8104, F-75014 Paris, France., Capitao M; Université de Paris, Institut Cochin, INSERM, U1016, CNRS, UMR 8104, F-75014 Paris, France., Barrin S; Université de Paris, Institut Cochin, INSERM, U1016, CNRS, UMR 8104, F-75014 Paris, France., Amziani S; Université de Paris, Institut Cochin, INSERM, U1016, CNRS, UMR 8104, F-75014 Paris, France., Pierre Paul P; Université de Paris, Institut Cochin, INSERM, U1016, CNRS, UMR 8104, F-75014 Paris, France., Borreill S; Université de Paris, Institut Cochin, INSERM, U1016, CNRS, UMR 8104, F-75014 Paris, France., Guilbert T; Université de Paris, Institut Cochin, INSERM, U1016, CNRS, UMR 8104, F-75014 Paris, France., Donnadieu E; Université de Paris, Institut Cochin, INSERM, U1016, CNRS, UMR 8104, F-75014 Paris, France., Niedergang F; Université de Paris, Institut Cochin, INSERM, U1016, CNRS, UMR 8104, F-75014 Paris, France., Ouaaz F; Université de Paris, Institut Cochin, INSERM, U1016, CNRS, UMR 8104, F-75014 Paris, France fatah.ouaaz@inserm.fr.
Jazyk:	angličtina
Zdroj:	Journal of immunology (Baltimore, Md. : 1950) [J Immunol] 2020 Aug 01; Vol. 205 (3), pp. 608-618. Date of Electronic Publication: 2020 Jun 24.
DOI:	10.4049/jimmunol.1900394
Abstrakt:	Dendritic cells (DCs) are professional APCs, which sample Ags in the periphery and migrate to the lymph node where they activate T cells. DCs can also present native Ag to B cells through interactions observed both in vitro and in vivo. However, the mechanisms of Ag transfer and B cell activation by DCs remain incompletely understood. In this study, we report that murine DCs are an important cell transporter of Ag from the periphery to the lymph node B cell zone and also potent inducers of B cell activation both in vivo and in vitro. Importantly, we highlight a novel extracellular mechanism of B cell activation by DCs. In this study, we demonstrate that Ag released upon DC regurgitation is sufficient to efficiently induce early B cell activation, which is BCR driven and mechanistically dependent on the nuclear accumulation of the transcription factor NF-κB/cRel. Thus, our study provides new mechanistic insights into Ag delivery and B cell activation modalities by DCs and a promising approach for targeting NF-κB/cRel pathway to modulate the DC-elicited B cell responses. (Copyright © 2020 by The American Association of Immunologists, Inc.)
Databáze:	MEDLINE
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