| Autor: |
Chaubet L; Department of Bioengineering, McGill University, Montreal, QC H3A 0C3, Canada., Chaudhary AR; Department of Bioengineering, McGill University, Montreal, QC H3A 0C3, Canada., Heris HK; Department of Bioengineering, McGill University, Montreal, QC H3A 0C3, Canada., Ehrlicher AJ; Department of Bioengineering, McGill University, Montreal, QC H3A 0C3, Canada., Hendricks AG; Department of Bioengineering, McGill University, Montreal, QC H3A 0C3, Canada. |
| Abstrakt: |
Cells precisely control their mechanical properties to organize and differentiate into tissues. The architecture and connectivity of cytoskeletal filaments change in response to mechanical and biochemical cues, allowing the cell to rapidly tune its mechanics from highly cross-linked, elastic networks to weakly cross-linked viscous networks. While the role of actin cross-linking in controlling actin network mechanics is well-characterized in purified actin networks, its mechanical role in the cytoplasm of living cells remains unknown. Here, we probe the frequency-dependent intracellular viscoelastic properties of living cells using multifrequency excitation and in situ optical trap calibration. At long timescales in the intracellular environment, we observe that the cytoskeleton becomes fluid-like. The mechanics are well-captured by a model in which actin filaments are dynamically connected by a single dominant cross-linker. A disease-causing point mutation (K255E) of the actin cross-linker α-actinin 4 (ACTN4) causes its binding kinetics to be insensitive to tension. Under normal conditions, the viscoelastic properties of wild-type (WT) and K255E+/- cells are similar. However, when tension is reduced through myosin II inhibition, WT cells relax 3× faster to the fluid-like regime while K255E+/- cells are not affected. These results indicate that dynamic actin cross-linking enables the cytoplasm to flow at long timescales. |
