Enteric viruses circulating in undiagnosed central nervous system infections at tertiary hospital in São José do Rio Preto, São Paulo, Brazil.

Autor: Rocha LCD; São José do Rio Preto School of Medicine (FAMERP), São Paulo, Brazil., Estofolete CF; São José do Rio Preto School of Medicine (FAMERP), São Paulo, Brazil., Milhim BHGA; São José do Rio Preto School of Medicine (FAMERP), São Paulo, Brazil., Augusto MT; São José do Rio Preto School of Medicine (FAMERP), São Paulo, Brazil., Zini N; São José do Rio Preto School of Medicine (FAMERP), São Paulo, Brazil., Silva GCDD; São José do Rio Preto School of Medicine (FAMERP), São Paulo, Brazil., Ferraz-Junior HC; São José do Rio Preto School of Medicine (FAMERP), São Paulo, Brazil., Brienze VMS; São José do Rio Preto Base Hospital, São Paulo, Brazil., Liso E; São José do Rio Preto Base Hospital, São Paulo, Brazil., Cunha MS; Vector Borne Disease Laboratory, Virology Center, Adolfo Lutz Institute, São Paulo, Brazil., Sabino EC; LIM/46, Faculty of Medicine, University of São Paulo, São Paulo, Brazil.; Institute of Tropical Medicine, University of São Paulo, São Paulo, Brazil., da Costa AC; Institute of Tropical Medicine, University of São Paulo, São Paulo, Brazil., Nogueira ML; São José do Rio Preto School of Medicine (FAMERP), São Paulo, Brazil., Luchs A; Enteric Disease Laboratory, Virology Center, Adolfo Lutz Institute, São Paulo, Brazil., Terzian ACB; São José do Rio Preto School of Medicine (FAMERP), São Paulo, Brazil.
Jazyk: angličtina
Zdroj: Journal of medical virology [J Med Virol] 2021 Jun; Vol. 93 (6), pp. 3539-3548. Date of Electronic Publication: 2020 Jul 11.
DOI: 10.1002/jmv.26216
Abstrakt: Enterovirus (EV) is commonly associated with central nervous system (CNS) syndromes. Recently, gastroenteric viruses, including rotavirus (RVA), human astrovirus (HAstV), and norovirus (NoV), have also been associated with CNS neurological disorders. The aim of the present study was to investigate the presence of EV, RVA, HAst, and NoV associated to CNS infections with undiagnosed etiology in Northwest region of São Paulo State, Brazil, and to conduct the molecular characterization of the positive samples detected. A total of 288 cerebrospinal fluid samples collected from July to December 2017 were tested for EV and NoV by quantitative real-time polymerase chain reaction (RT-qPCR), HAstV by conventional RT-PCR, and RVA by enzyme-linked immunosorbent assay. Positive-EV samples were inoculated in cells lines, amplified by RT-PCR and sequenced. RVA, NoV, and HAstV were not detected. EV infection was detected in 5.5% (16/288), and five samples successful genotyped: echovirus 3 (E3) (1/5), coxsackie virus A6 (CVA6) (1/5), and coxsackie virus B4 (CVB4) (3/5). Meningitis was the main syndrome observed (12/16; 75%). CVA6, CVB4, and E3 were identified associated with aseptic meningitis. Reports of CVA6 associated with aseptic meningitis are rare, E3 had not been previously reported in Brazil, and epidemiological data on CVB4 in the country is virtually unknown. The present investigation illustrates the circulation of diverse EV types in a small regional sample set and in a short period of time, highlighting the importance of an active EV surveillance system in CNS infections. Enhanced understanding of undiagnosed CNS infections will assist in public health and health care planning.
(© 2020 Wiley Periodicals LLC.)
Databáze: MEDLINE
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