Diagnostic accuracy of 18F-FDG-PET in abdominal sepsis in rats.

Autor:	Azevedo ÍM; Fellow PhD degree, Postgraduate Program in Health Sciences, Universidade Federal do Rio Grande do Norte (UFRN), Natal-RN, Brazil. Technical procedures, acquisition of data, statistics analysis, critical revision., Macedo Filho R; Fellow PhD degree, Postgraduate Program in Health Sciences, Universidade Federal do Rio Grande do Norte (UFRN), Natal-RN, Brazil. Technical procedures, acquisition of data, statistics analysis, critical revision., Rocha KBF; Fellow PhD degree, Postgraduate Program in Health Sciences, Universidade Federal do Rio Grande do Norte (UFRN), Natal-RN, Brazil. Technical procedures, acquisition of data, statistics analysis, critical revision., Oliveira CN; Fellow PhD degree, Postgraduate Program in Health Sciences, Universidade Federal do Rio Grande do Norte (UFRN), Natal-RN, Brazil. Technical procedures, acquisition of data, statistics analysis, critical revision., Medeiros AC; PhD, Full Professor, Nucleus of Experimental Surgery, UFRN, Natal-RN, Brazil. Conception, design, intellectual and scientific content of the study; critical revision; final approval.
Jazyk:	angličtina
Zdroj:	Acta cirurgica brasileira [Acta Cir Bras] 2020 Jun 22; Vol. 35 (5), pp. e202000505. Date of Electronic Publication: 2020 Jun 22 (Print Publication: 2020).
DOI:	10.1590/s0102-865020200050000005
Abstrakt:	Purpose: The objective of this study was to investigate the accuracy of 18F-FDG-PET in the diagnosis of multibacterial abdominal sepsis by cecum ligation and puncture (CLP) in rats. Methods: Adult Wistar rats ( Rattus norvegicus ), weighing 227±35g, were allocated into a sepsis group by CLP (n=10) and sham group (n=10). 18F-FDG-PET using microPET was performed on all rats after 24 hours. Results: All animals survived for postoperative 24h. The abdomen/liver ratio of the standardized uptake value (SUV) percentage was significantly higher in the sepsis group than in the sham (p=0.004). The ROC curve showed an accuracy of 18F-FDG-PET to detect abdominal sepsis of 88.9% (p=0.001), sensitivity of 90% and specificity of 88.9%. When a cut-off point of 79% of the ratio between the SUV on the abdominal region and liver was established, the sensitivity was 90%, specificity of 88.9%; positive and negative predictive values of 90.0% and 88.9%, respectively. Conclusions: The diagnostic accuracy of 18F-FDG-PET in rats with abdominal sepsis was significantly high. It was also demonstrated the predictive ability of the abdomen/liver SUV ratio to diagnose abdominal sepsis. These findings may have implications for the clinical setting, locating septic foci with PETscan.
Databáze:	MEDLINE
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