An enhanced isothermal amplification assay for viral detection.
| Autor: | Qian J, Boswell SA, Chidley C, Lu ZX, Pettit ME, Gaudio BL, Fajnzylber JM, Ingram RT, Ward RH, Li JZ, Springer M |
|---|---|
| Jazyk: | angličtina |
| Zdroj: | BioRxiv : the preprint server for biology [bioRxiv] 2020 May 29. Date of Electronic Publication: 2020 May 29. |
| DOI: | 10.1101/2020.05.28.118059 |
| Abstrakt: | Rapid, inexpensive, robust diagnostics are essential to control the spread of infectious diseases. Current state of the art diagnostics are highly sensitive and specific, but slow, and require expensive equipment. We developed a molecular diagnostic test for SARS-CoV-2, FIND (Fast Isothermal Nucleic acid Detection), based on an enhanced isothermal recombinase polymerase amplification reaction. FIND has a detection limit on patient samples close to that of RT-qPCR, requires minimal instrumentation, and is highly scalable and cheap. It can be performed in high throughput, does not cross-react with other common coronaviruses, avoids bottlenecks caused by the current worldwide shortage of RNA isolation kits, and takes ~45 minutes from sample collection to results. FIND can be adapted to future novel viruses in days once sequence is available. One Sentence Summary: Sensitive, specific, rapid, scalable, enhanced isothermal amplification method for detecting SARS-CoV-2 from patient samples. |
| Databáze: | MEDLINE |
| Externí odkaz: |
|