DEPDC5 haploinsufficiency drives increased mTORC1 signaling and abnormal morphology in human iPSC-derived cortical neurons.

Autor: Klofas LK; Vanderbilt Brain Institute, Vanderbilt University, Nashville, TN, USA., Short BP; Department of Pediatrics, Vanderbilt University Medical Center, Nashville, TN, USA., Snow JP; Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, TN, USA., Sinnaeve J; Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, TN, USA., Rushing GV; Vanderbilt Brain Institute, Vanderbilt University, Nashville, TN, USA., Westlake G; Department of Pediatrics, Vanderbilt University Medical Center, Nashville, TN, USA., Weinstein W; Department of Pediatrics, Vanderbilt University Medical Center, Nashville, TN, USA., Ihrie RA; Vanderbilt Brain Institute, Vanderbilt University, Nashville, TN, USA; Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, TN, USA; Department of Neurological Surgery, Vanderbilt University Medical Center, Nashville, TN, USA., Ess KC; Vanderbilt Brain Institute, Vanderbilt University, Nashville, TN, USA; Department of Pediatrics, Vanderbilt University Medical Center, Nashville, TN, USA; Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, TN, USA., Carson RP; Vanderbilt Brain Institute, Vanderbilt University, Nashville, TN, USA; Department of Pediatrics, Vanderbilt University Medical Center, Nashville, TN, USA; Department of Pharmacology, Vanderbilt University Medical Center, Nashville, TN, USA. Electronic address: robert.carson@vumc.org.
Jazyk: angličtina
Zdroj: Neurobiology of disease [Neurobiol Dis] 2020 Sep; Vol. 143, pp. 104975. Date of Electronic Publication: 2020 Jun 20.
DOI: 10.1016/j.nbd.2020.104975
Abstrakt: Mutations in the DEPDC5 gene can cause epilepsy, including forms with and without brain malformations. The goal of this study was to investigate the contribution of DEPDC5 gene dosage to the underlying neuropathology of DEPDC5-related epilepsies. We generated induced pluripotent stem cells (iPSCs) from epilepsy patients harboring heterozygous loss of function mutations in DEPDC5. Patient iPSCs displayed increases in both phosphorylation of ribosomal protein S6 and proliferation rate, consistent with elevated mTORC1 activation. In line with these findings, we observed increased soma size in patient iPSC-derived cortical neurons that was rescued with rapamycin treatment. These data indicate that human cells heterozygous for DEPDC5 loss-of-function mutations are haploinsufficient for control of mTORC1 signaling. Our findings suggest that human pathology differs from mouse models of DEPDC5-related epilepsies, which do not show consistent phenotypic differences in heterozygous neurons, and support the need for human-based models to affirm and augment the findings from animal models of DEPDC5-related epilepsy.
(Copyright © 2020 The Authors. Published by Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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