Relationship between apical periodontitis and atherosclerosis in rats: lipid profile and histological study.

Autor: Conti LC; Endodontic Section, Department of Preventive and Restorative Dentistry, School of Dentistry, São Paulo State University (Unesp), Araçatuba, SP, Brazil., Segura-Egea JJ; Endodontic Section, Department of Stomatology, School of Dentistry, University of Sevilla, Sevilla, Spain., Cardoso CBM; Endodontic Section, Department of Preventive and Restorative Dentistry, School of Dentistry, São Paulo State University (Unesp), Araçatuba, SP, Brazil., Benetti F; Endodontic Section, Department of Restorative Dentistry, School of Dentistry, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil., Azuma MM; Department of Cariology, Restorative Sciences and Endodontics, University of Michigan, Ann Arbor, MI, USA., Oliveira PHC; Endodontic Section, Department of Preventive and Restorative Dentistry, School of Dentistry, São Paulo State University (Unesp), Araçatuba, SP, Brazil., Bomfim SRM; Department Clinic and Surgery and Animal Reproduction, Araçatuba Veterinary Medicine, São Paulo State University (Unesp), Araçatuba, SP, Brazil., Cintra LTA; Endodontic Section, Department of Preventive and Restorative Dentistry, School of Dentistry, São Paulo State University (Unesp), Araçatuba, SP, Brazil.
Jazyk: angličtina
Zdroj: International endodontic journal [Int Endod J] 2020 Oct; Vol. 53 (10), pp. 1387-1397. Date of Electronic Publication: 2020 Jul 16.
DOI: 10.1111/iej.13350
Abstrakt: Aim: To investigate the relationship between apical periodontitis and atherosclerosis in rats by lipid profile and carotid artery intima tunic measurement, and histological and histometric evaluation of periapical lesions.
Methodology: Forty male Wistar rats were allocated into four groups: control (C), with apical periodontitis (AP), with atherosclerosis (AT) and with AP and AT (AP + AT). Atherosclerosis was induced using a high-lipid diet associated with a surgical ligature in the carotid artery and a super dosage of vitamin D 3 . AP was induced via pulp exposure to the oral environment. At 45 and 75 days, serum levels of total cholesterol (TC), triglycerides (TG), high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C) were measured. The maxillary and mandibular jaws and carotid artery were collected and processed for histological analysis. The Kruskal-Wallis or Mann-Whitney test was performed for nonparametric data, and the Tukey's or Student's t-test was performed for parametric data (P < 0.05).
Results: In nonatherosclerotic animals, the induction of apical periodontitis increased TG levels significantly, from 63.1 ± 11.4 mg dL -1 in group C to 88.2 ± 7.9 mg dL -1 in the AP group (P < 0.05). The induction of AP was associated with a trend for higher TC and LDL-C levels in atherosclerotic animals (P > 0.05); however, it only significantly increased TG levels, from 93.2 ± 18.0 mg dL -1 in AT group to 121.9 ± 14.5 mg dL -1 in the AP + AT group (P < 0.05). Animals in the AP + AT group had a 36.5% increase in the thickness of the carotid intima tunic when compared with the AT group (P < 0.05). The intensity of the inflammatory infiltrate was significantly larger in the AP + AT group when compared with AP group (P < 0.05). The AP + AT group exhibited significantly greater alveolar bone loss, with a periapical lesion size of 206.4 ± 56.3 × 10 4 μm 2 , compared with 151.4 ± 49.1 × 10 4 μm 2 in the AP group (P < 0.05).
Conclusion: Apical periodontitis influenced triglyceride levels, increasing them even in the absence of atherosclerosis, and influenced the increase in the thickness of the carotid artery intima tunic in the presence of atherosclerosis. Atherosclerosis intensified the inflammatory reaction and increased bone resorption in periapical lesions.
(© 2020 International Endodontic Journal. Published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
Externí odkaz: