The E2 Marie Kondo and the CTLH E3 ligase clear deposited RNA binding proteins during the maternal-to-zygotic transition.
| Autor: | Zavortink M; University of Colorado School of Medicine, Aurora, United States., Rutt LN; University of Colorado School of Medicine, Aurora, United States., Dzitoyeva S; University of Colorado School of Medicine, Aurora, United States., Henriksen JC; University of Colorado School of Medicine, Aurora, United States., Barrington C; University of Colorado School of Medicine, Aurora, United States., Bilodeau DY; University of Colorado School of Medicine, Aurora, United States., Wang M; University of Toronto, Toronto, Canada., Chen XXL; University of Toronto, Toronto, Canada., Rissland OS; University of Colorado School of Medicine, Aurora, United States. |
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| Jazyk: | angličtina |
| Zdroj: | ELife [Elife] 2020 Jun 23; Vol. 9. Date of Electronic Publication: 2020 Jun 23. |
| DOI: | 10.7554/eLife.53889 |
| Abstrakt: | The maternal-to-zygotic transition (MZT) is a conserved step in animal development, where control is passed from the maternal to the zygotic genome. Although the MZT is typically considered from its impact on the transcriptome, we previously found that three maternally deposited Drosophila RNA-binding proteins (ME31B, Trailer Hitch [TRAL], and Cup) are also cleared during the MZT by unknown mechanisms. Here, we show that these proteins are degraded by the ubiquitin-proteasome system. Marie Kondo, an E2 conjugating enzyme, and the E3 CTLH ligase are required for the destruction of ME31B, TRAL, and Cup. Structure modeling of the Drosophila CTLH complex suggests that substrate recognition is different than orthologous complexes. Despite occurring hours earlier, egg activation mediates clearance of these proteins through the Pan Gu kinase, which stimulates translation of Kdo mRNA. Clearance of the maternal protein dowry thus appears to be a coordinated, but as-yet underappreciated, aspect of the MZT. Competing Interests: MZ, LR, SD, JH, CB, DB, MW, XC, OR No competing interests declared (© 2020, Zavortink et al.) |
| Databáze: | MEDLINE |
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