Non-invasive monitoring of chronic liver disease via near-infrared and shortwave-infrared imaging of endogenous lipofuscin.

Autor: Saif M; Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA, USA., Kwanten WJ; Edwin L. Steele Laboratories of Tumor Biology, Department of Radiation Oncology, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA.; Laboratory of Experimental Medicine and Pediatrics (LEMP)-Gastroenterology and Hepatology, University of Antwerp, Wilrijk, Belgium., Carr JA; Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA, USA., Chen IX; Edwin L. Steele Laboratories of Tumor Biology, Department of Radiation Oncology, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA., Posada JM; Edwin L. Steele Laboratories of Tumor Biology, Department of Radiation Oncology, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA.; Department of Pathology, Brigham and Women's Hospital, Boston, MA, USA., Srivastava A; Department of Pathology, Brigham and Women's Hospital, Boston, MA, USA., Zhang J; Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA, USA., Zheng Y; Neuroprotection Research Laboratory, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA, USA., Pinter M; Edwin L. Steele Laboratories of Tumor Biology, Department of Radiation Oncology, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA.; Division of Gastroenterology and Hepatology, Department of Internal Medicine III, Medical University of Vienna, Vienna, Austria., Chatterjee S; Edwin L. Steele Laboratories of Tumor Biology, Department of Radiation Oncology, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA., Softic S; Joslin Diabetes Center, Harvard Medical School, Boston, MA, USA.; Division of Gastroenterology, Boston Children's Hospital, Boston, MA, USA.; Division of Gastroenterology, Hepatology and Nutrition, Department of Pediatrics, University of Kentucky College of Medicine and Kentucky Children's Hospital, Lexington, KY, USA., Kahn CR; Joslin Diabetes Center, Harvard Medical School, Boston, MA, USA., van Leyen K; Neuroprotection Research Laboratory, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA, USA., Bruns OT; Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA, USA.; Helmholtz Pioneer Campus, Helmholtz Zentrum München, Neuherberg, Germany., Jain RK; Edwin L. Steele Laboratories of Tumor Biology, Department of Radiation Oncology, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA. jain@steele.mgh.harvard.edu., Bawendi MG; Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA, USA. mgb@mit.edu.
Jazyk: angličtina
Zdroj: Nature biomedical engineering [Nat Biomed Eng] 2020 Aug; Vol. 4 (8), pp. 801-813. Date of Electronic Publication: 2020 Jun 22.
DOI: 10.1038/s41551-020-0569-y
Abstrakt: Monitoring the progression of non-alcoholic fatty liver disease is hindered by a lack of suitable non-invasive imaging methods. Here, we show that the endogenous pigment lipofuscin displays strong near-infrared and shortwave-infrared fluorescence when excited at 808 nm, enabling label-free imaging of liver injury in mice and the discrimination of pathological processes from normal liver processes with high specificity and sensitivity. We also show that the near-infrared and shortwave-infrared fluorescence of lipofuscin can be used to monitor the progression and regression of liver necroinflammation and fibrosis in mouse models of non-alcoholic fatty liver disease and advanced fibrosis, as well as to detect non-alcoholic steatohepatitis and cirrhosis in biopsied samples of human liver tissue.
Databáze: MEDLINE