Patterns of alveolar macrophage activation upon attenuated and virulent African swine fever viruses in vitro.

Autor: Tatoyan MR; Yerevan State Medical University, Yerevan, Armenia., Izmailyan RA; Laboratory of Cell Biology and Virology, Institute of Molecular Biology of NAS RA, Yerevan, Armenia., Semerjyan AB; Yerevan State Medical University, Yerevan, Armenia., Karalyan NY; Yerevan State Medical University, Yerevan, Armenia., Sahakyan CT; Yerevan State Medical University, Yerevan, Armenia., Mkrtchyan GL; Yerevan State Medical University, Yerevan, Armenia., Ghazaryan HK; Laboratory of Human Genomics and Immunomics, Institute of Molecular Biology of NAS RA, Yerevan, Armenia., Arzumanyan HH; Laboratory of Cell Biology and Virology, Institute of Molecular Biology of NAS RA, Yerevan, Armenia., Semerjyan ZB; Laboratory of Cell Biology and Virology, Institute of Molecular Biology of NAS RA, Yerevan, Armenia; Experimental Laboratory, Yerevan State Medical University, Yerevan, Armenia., Karalova EM; Laboratory of Cell Biology and Virology, Institute of Molecular Biology of NAS RA, Yerevan, Armenia; Experimental Laboratory, Yerevan State Medical University, Yerevan, Armenia., Karalyan ZA; Laboratory of Cell Biology and Virology, Institute of Molecular Biology of NAS RA, Yerevan, Armenia; Yerevan State Medical University, Yerevan, Armenia. Electronic address: zkaralyan@yahoo.com.
Jazyk: angličtina
Zdroj: Comparative immunology, microbiology and infectious diseases [Comp Immunol Microbiol Infect Dis] 2020 Oct; Vol. 72, pp. 101513. Date of Electronic Publication: 2020 Jun 16.
DOI: 10.1016/j.cimid.2020.101513
Abstrakt: The pattern of porcine alveolar macrophage (AM) activation upon classical stimuli of two strains of African swine fever (ASF) viruses, an attenuated ASFV-BA71V and virulent ASFV-Georgia2007 were investigated. In an in vitro experiment ASFV-Georgia2007-infected AM showed M1 polarization pattern different from the one induced by classical stimuli. Altered morphology, appearance of binuclear cells, decreased synthesis of IFN-alpha as well as IFN-epsilon was observed compared with attenuated ASFV-BA71V, and decreased synthesis of IFN-omega compared with intact cells. However, CD68 level did not significantly differ between alveolar macrophage populations infected by ASFV-Georgia2007 and control group, while both LPS/IFN-gamma stimulation and non-pathogenic ASFV-BA71V virus increased the level of CD68 soluble receptor. AM infection with ASFV-Georgia2007 resulted in remarkable DNA proliferation whereas LPS/IFN-gamma and ASFV-BA71V induced less expressed DNA proliferation in activated cells. The higher value of nitric oxide was obvious in the cells infected with ASFV-BA71V, compared to ASFV-Georgia2007 and LPS/IFN-gamma activated cells. In conclusion, pattern of activation of alveolar macrophages induced by ASFV-Georgia2007 virus differs from the one expressed in LPS/IFN-gamma- and ASFV-BA71V-activated cells. ASFV-BA71V and LPS/IFN-gamma share similar antiviral response of porcine AM. Therefore we assume that wild type virulent ASFV can partially down regulate antiviral response of AM and conclude that evolutionary decrease of virulence in ASFV is related to alterations of control of the host cell antiviral response.
(Copyright © 2020 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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