A divergent cyclin/cyclin-dependent kinase complex controls the atypical replication of a malaria parasite during gametogony and transmission.

Autor:	Balestra AC; University of Geneva, Faculty of Medicine, Geneva, Switzerland., Zeeshan M; University of Nottingham, School of Life Sciences, Nottingham, United Kingdom., Rea E; University of Nottingham, School of Life Sciences, Nottingham, United Kingdom., Pasquarello C; University of Geneva, Faculty of Medicine, Geneva, Switzerland., Brusini L; University of Geneva, Faculty of Medicine, Geneva, Switzerland.; University of Nottingham, School of Life Sciences, Nottingham, United Kingdom., Mourier T; Biological and Environmental Sciences and Engineering (BESE) Division, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia., Subudhi AK; Biological and Environmental Sciences and Engineering (BESE) Division, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia., Klages N; University of Geneva, Faculty of Medicine, Geneva, Switzerland., Arboit P; University of Geneva, Faculty of Medicine, Geneva, Switzerland., Pandey R; University of Nottingham, School of Life Sciences, Nottingham, United Kingdom., Brady D; University of Nottingham, School of Life Sciences, Nottingham, United Kingdom., Vaughan S; Oxford Brookes University, Department of Biological and Medical Sciences, Oxford, United Kingdom., Holder AA; The Francis Crick Institute, Malaria Parasitology Laboratory, London, United Kingdom., Pain A; Biological and Environmental Sciences and Engineering (BESE) Division, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia., Ferguson DJ; Oxford Brookes University, Department of Biological and Medical Sciences, Oxford, United Kingdom.; University of Oxford, John Radcliffe Hospital, Nuffield Department of Clinical Laboratory Science, Oxford, United Kingdom., Hainard A; University of Geneva, Faculty of Medicine, Geneva, Switzerland., Tewari R; University of Nottingham, School of Life Sciences, Nottingham, United Kingdom., Brochet M; University of Geneva, Faculty of Medicine, Geneva, Switzerland.
Jazyk:	angličtina
Zdroj:	ELife [Elife] 2020 Jun 22; Vol. 9. Date of Electronic Publication: 2020 Jun 22.
DOI:	10.7554/eLife.56474
Abstrakt:	Cell cycle transitions are generally triggered by variation in the activity of cyclin-dependent kinases (CDKs) bound to cyclins. Malaria-causing parasites have a life cycle with unique cell-division cycles, and a repertoire of divergent CDKs and cyclins of poorly understood function and interdependency. We show that Plasmodium berghei CDK-related kinase 5 (CRK5), is a critical regulator of atypical mitosis in the gametogony and is required for mosquito transmission. It phosphorylates canonical CDK motifs of components in the pre-replicative complex and is essential for DNA replication. During a replicative cycle, CRK5 stably interacts with a single Plasmodium -specific cyclin (SOC2), although we obtained no evidence of SOC2 cycling by transcription, translation or degradation. Our results provide evidence that during Plasmodium male gametogony, this divergent cyclin/CDK pair fills the functional space of other eukaryotic cell-cycle kinases controlling DNA replication. Competing Interests: AB, MZ, ER, CP, LB, TM, AS, NK, PA, RP, DB, SV, AH, AP, DF, AH, RT, MB No competing interests declared (© 2020, Balestra et al.)
Databáze:	MEDLINE
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