GDNF promotes hair formation and cutaneous wound healing by targeting bulge stem cells.

Autor:	Lisse TS; The Jackson Laboratory, 600 Main Street, Bar Harbor, ME 04609 USA.; Department of Biology, The University of Miami, 1301 Memorial Drive, Cox Science Building, Coral Gables, FL 33124 USA.; Sylvester Comprehensive Cancer Center, Miller School of Medicine, University of Miami, Miami, FL 33146 USA., Sharma M; The Jackson Laboratory, 600 Main Street, Bar Harbor, ME 04609 USA., Vishlaghi N; Department of Biology, The University of Miami, 1301 Memorial Drive, Cox Science Building, Coral Gables, FL 33124 USA., Pullagura SR; The Jackson Laboratory, 600 Main Street, Bar Harbor, ME 04609 USA.; Graduate School of Biomedical Sciences and Engineering, University of Maine, Orono, 04469 ME USA., Braun RE; The Jackson Laboratory, 600 Main Street, Bar Harbor, ME 04609 USA.; Graduate School of Biomedical Sciences and Engineering, University of Maine, Orono, 04469 ME USA.
Jazyk:	angličtina
Zdroj:	NPJ Regenerative medicine [NPJ Regen Med] 2020 Jun 12; Vol. 5, pp. 13. Date of Electronic Publication: 2020 Jun 12 (Print Publication: 2020).
DOI:	10.1038/s41536-020-0098-z
Abstrakt:	Glial-cell-derived neurotrophic factor (GDNF) is a well-studied neuroregenerative factor; however, the degree to which it supports hair formation and skin wound repair is not known. By using a Gfra1 (GDNF family receptor alpha 1) knock-in reporter mouse line, GDNF signaling was found to occur within hair bulge stem cells (BSCs) during the initiation of the hair cycle and early stages of hair formation after depilation. Both recombinant and transgene overexpression of GDNF promoted BSC colony growth, hair formation, and skin repair after wounding through enhanced self-renewal of BSCs and commitment of BSC-derived progenitors into becoming epidermal cells at the injury site. Conditional ablation of Gfra1 among BSCs impaired the onset of the hair cycle, while conditional ablation of the GDNF family member signal transducer, Ret , within BSCs prevented the onset of the hair cycle and depilation-induced anagen development of hair follicles. Our findings reveal that GDNF promotes hair formation and wound repair and that bulge stem cells are critical mediators of both. Competing Interests: Competing interestsThe authors declare no competing interests. (© The Author(s) 2020.)
Databáze:	MEDLINE
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