A semi-synthetic flavonoid from Bauhinia pulchella stem attenuates inflammatory osteolysis in periodontitis in rats: Impact on cytokine levels, oxidative stress, and RANK/RANKL/OPG pathway.

Autor: Pinto IR; Northeast Biotechnology Network - Ph.D. Program, Federal University of Ceará, Brazil., Chaves HV; School of Dentistry, Federal University of Ceará, Sobral, Ceará, Brazil., Freire JMO; Northeast Biotechnology Network - Ph.D. Program, Federal University of Ceará, Brazil., de Sousa LHT; Northeast Biotechnology Network - Ph.D. Program, Federal University of Ceará, Brazil., Monteiro DAM; School of Medicine, Federal University of Ceará, Sobral, Ceará, Brazil., Costa JJDN; School of Medicine, University Center INTA - UNINTA, Sobral, Ceará, Brazil., Pereira KMA; Department of Morphology, School of Medicine, Federal University of Ceará, Fortaleza, Ceará, Brazil., Santiago GMP; Postgraduate Program in Chemistry, Federal University of Ceará, Fortaleza, Ceará, Brazil., de Sousa LM; Postgraduate Program in Chemistry, Federal University of Ceará, Fortaleza, Ceará, Brazil., da Silva MR; Postgraduate Program in Chemistry, Federal University of Ceará, Fortaleza, Ceará, Brazil., Monteiro AO; Postgraduate Program in Chemistry, Federal University of Ceará, Fortaleza, Ceará, Brazil., Montenegro RC; Drug Research and Development Center (NPDM), Federal University of Ceará, Fortaleza, Ceará, Brazil., de Moraes MEA; Drug Research and Development Center (NPDM), Federal University of Ceará, Fortaleza, Ceará, Brazil., Filho GC; School of Medicine, Federal University of Ceará, Sobral, Ceará, Brazil., Pinto VPT; School of Medicine, Federal University of Ceará, Sobral, Ceará, Brazil., Bezerra MM; School of Medicine, Federal University of Ceará, Sobral, Ceará, Brazil; Drug Research and Development Center (NPDM), Federal University of Ceará, Fortaleza, Ceará, Brazil. Electronic address: mirna@ufc.br.
Jazyk: angličtina
Zdroj: Archives of oral biology [Arch Oral Biol] 2020 Sep; Vol. 117, pp. 104816. Date of Electronic Publication: 2020 Jun 10.
DOI: 10.1016/j.archoralbio.2020.104816
Abstrakt: Objectives: Many species of theBauhinia genus have been widely used in folk medicine as analgesic, anti-inflammatory and antioxidant agents. (-)-Fisetinidol palmitate is a semi-syntetic flavonoid obtained from the ethanolic extract of the stem of Bauhinia pulchella. This study aimed to evaluate the antiresorptive effect of the semi-syntetic (-)-fisetinidol palmitate in ligature-induced periodontitis in rats. Also, it evaluated the mechanism of action of (-)-fisetinidol palmitate and its toxicity.
Design: Periodontitis was inducedvia a nylon thread ligature (3.0) around the second upper left molars. Rats were treated (oral gavage) once a day for 11 days with (-)-fisetinidol palmitate (0.01 or 0.1 mg/kg) or saline vehicle.
Results: (-)-Fisetinidol palmitate (0.1 mg/kg) reduced alveolar bone loss, increased bone alkaline phosphatase (BALP), superoxide dismutase (SOD), and catalase (CAT) activity; also, it decreased IL1-β, IL-8/CINC-1, nitrite/nitrate levels and myeloperoxidase activity. (-)-Fisetinidol palmitate reduced the mRNA levels of IL1-β, IL-6, RANK, and RANK-L, while it increased the OPG ones. No statistical differences (P > 0.05) were observed in the transaminases (ALT, AST) and Total Alkaline Phosphatase (TALP) levels among groups.
(-)- Conclusions: Fisetinidol palmitate did not result in any signs of toxicity and had anti-resorptive effects in a pre-clinical trial of periodontitis, showing antioxidant activity with the involvement of the RANK/RANKL/OPG pathway.
Competing Interests: Declaration of Competing Interest The authors declare that they have no conflict of interest.
(Copyright © 2020 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: