NMR-based metabolic profiling to follow the production of anti-phytopathogenic compounds in the culture of the marine strain Streptomyces sp. PNM-9.

Autor: Betancur LA; Universidad Nacional de Colombia, Sede Bogotá, Departamento de Química, Carrera 30 No. 45-03, Edificio de Química of 427, Bogotá, 111321, Colombia; Universidad de Caldas, Departamento de Química, Edificio Orlando Sierra, Bloque B, Sede Palogrande Calle 65 No. 26-10. Manizales, Caldas, Colombia., Forero AM; Universidad Nacional de Colombia, Sede Bogotá, Departamento de Química, Carrera 30 No. 45-03, Edificio de Química of 427, Bogotá, 111321, Colombia., Vinchira-Villarraga DM; Universidad Nacional de Colombia, Sede Bogotá, Departamento de Química, Carrera 30 No. 45-03, Edificio de Química of 427, Bogotá, 111321, Colombia., Cárdenas JD; Universidad Nacional de Colombia, Sede Bogotá, Departamento de Química, Carrera 30 No. 45-03, Edificio de Química of 427, Bogotá, 111321, Colombia., Romero-Otero A; Universidad Nacional de Colombia, Sede Bogotá, Departamento de Química, Carrera 30 No. 45-03, Edificio de Química of 427, Bogotá, 111321, Colombia., Chagas FO; Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Av. do Café, s/n, 14040-903; Ribeirão Preto, SP, Brazil., Pupo MT; Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Av. do Café, s/n, 14040-903; Ribeirão Preto, SP, Brazil., Castellanos L; Universidad Nacional de Colombia, Sede Bogotá, Departamento de Química, Carrera 30 No. 45-03, Edificio de Química of 427, Bogotá, 111321, Colombia., Ramos FA; Universidad Nacional de Colombia, Sede Bogotá, Departamento de Química, Carrera 30 No. 45-03, Edificio de Química of 427, Bogotá, 111321, Colombia. Electronic address: faramosr@unal.edu.co.
Jazyk: angličtina
Zdroj: Microbiological research [Microbiol Res] 2020 Oct; Vol. 239, pp. 126507. Date of Electronic Publication: 2020 Jun 03.
DOI: 10.1016/j.micres.2020.126507
Abstrakt: Actinobacteria are the major source of bioactive secondary metabolites and are featured in the search for antimicrobial compounds. We used nuclear magnetic resonance (RMN)-metabolic profiling and multivariate data analysis (MVDA) to correlate the metabolites' production of Streptomyces sp. PNM-9 from the algae Dictyota sp. and their biological activity against the rice phytopathogenic bacteria Burkholderia spp. The compounds 2-methyl-N-(2'-phenylethyl)-butanamide (1) and 3-methyl-N-(2'-phenylethyl)-butanamide (2) were identified through MVDA and 2D NMR experiments in the organic extract of a 15-days LB media culture of Streptomyces sp. PNM-9. Compounds 1 and 2 were isolated and their structures confirmed by one- and two-dimensional NMR and mass spectrometry (MS) data. Compounds 1 and 2 were active against the rice pathogenic bacteria Burkholderia glumae (ATCC 33,617) displaying minimal inhibitory concentration (MIC) values of 2.43 mM and 1.21 mM, respectively. The metabolomics-guided approach employing NMR-metabolic profiling was useful for marine microbial bioprospecting and suggested Streptomyces sp. PNM-9 strain and its compounds as a potential control against phytopathogenic bacteria.
(Copyright © 2020 Elsevier GmbH. All rights reserved.)
Databáze: MEDLINE
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