Lytic potential of Lysobacter capsici VKM B-2533 T : bacteriolytic enzymes and outer membrane vesicles.

Autor: Afoshin AS; Laboratory of Microbial Cell Surface Biochemistry, G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, PSCBR RAS, 5 Prosp. Nauki, Pushchino, Moscow Region, 142290, Russia., Kudryakova IV; Laboratory of Microbial Cell Surface Biochemistry, G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, PSCBR RAS, 5 Prosp. Nauki, Pushchino, Moscow Region, 142290, Russia., Borovikova AO; Laboratory of Microbial Cell Surface Biochemistry, G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, PSCBR RAS, 5 Prosp. Nauki, Pushchino, Moscow Region, 142290, Russia., Suzina NE; Laboratory of Microbial Cell Surface Biochemistry, G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, PSCBR RAS, 5 Prosp. Nauki, Pushchino, Moscow Region, 142290, Russia., Toropygin IY; Department of Proteomics, V.N. Orekhovich Research Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, 10 Pogodinskaja Str., Moscow, 119832, Russia., Shishkova NA; Laboratory of Anthrax Microbiology, FBIS State Research Center for Applied Microbiology and Biotechnology, Obolensk, Serpukhov District, Moscow Region, 142279, Russia., Vasilyeva NV; Laboratory of Microbial Cell Surface Biochemistry, G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, PSCBR RAS, 5 Prosp. Nauki, Pushchino, Moscow Region, 142290, Russia. vasilyevanv@rambler.ru.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2020 Jun 19; Vol. 10 (1), pp. 9944. Date of Electronic Publication: 2020 Jun 19.
DOI: 10.1038/s41598-020-67122-2
Abstrakt: Recent recurrent outbreaks of bacterial resistance to antibiotics have shown the critical need to identify new lytic agents to combat them. The species Lysobacter capsici VKM B-2533 T possesses a potent antimicrobial action against a number of bacteria, fungi and yeasts. Its activity can be due to the impact of bacteriolytic enzymes, antibiotics and peptides. This work isolated four homogeneous bacteriolytic enzymes and a mixture of two proteins, which also had a bacteriolytic activity. The isolates included proteins identical to L. enzymogenes α- and β-lytic proteases and lysine-specific protease. The proteases of 26 kDa and 29 kDa and a protein identified as N-acetylglycosaminidase had not been isolated in Lysobacter earlier. The isolated β-lytic protease digested live methicillin-resistant staphylococcal cells with high efficiency (minimal inhibitory concentration, 2.85 μg/mL). This property makes the enzyme deserving special attention. A recombinant β-lytic protease was produced. The antimicrobial potential of the bacterium was contributed to by outer membrane vesicles (OMVs). L. capsici cells were found to form a group of OMVs responsible for antifungal activity. The data are indicative of a significant antimicrobial potential of this bacterium that requires thorough research.
Databáze: MEDLINE
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