Is the orofacial antinociceptive effect of lectins intrinsically related to their specificity to monosaccharides?

Autor: de Oliveira Leite G; Núcleo de Biologia Experimental, Universidade de Fortaleza, Fortaleza, Brazil., Santos SAAR; Núcleo de Biologia Experimental, Universidade de Fortaleza, Fortaleza, Brazil., Bezerra FMDH; Núcleo de Biologia Experimental, Universidade de Fortaleza, Fortaleza, Brazil., Sena E Silva FE; Núcleo de Biologia Experimental, Universidade de Fortaleza, Fortaleza, Brazil., de Castro Ribeiro AD; Núcleo de Biologia Experimental, Universidade de Fortaleza, Fortaleza, Brazil., Roma RR; Centro de Ciências Agrárias e Ambientais, Universidade Federal do Maranhão, Chapadinha, Maranhão, Brazil., Silva RRS; Centro de Ciências Agrárias e Ambientais, Universidade Federal do Maranhão, Chapadinha, Maranhão, Brazil., Santos MHC; Centro de Ciências Agrárias e Ambientais, Universidade Federal do Maranhão, Chapadinha, Maranhão, Brazil., Santos ALE; Centro de Ciências Agrárias e Ambientais, Universidade Federal do Maranhão, Chapadinha, Maranhão, Brazil., Teixeira CS; Centro de Ciências Agrárias e Ambientais, Universidade Federal do Maranhão, Chapadinha, Maranhão, Brazil., Campos AR; Núcleo de Biologia Experimental, Universidade de Fortaleza, Fortaleza, Brazil. Electronic address: adrirolim@unifor.br.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2020 Oct 15; Vol. 161, pp. 1079-1085. Date of Electronic Publication: 2020 Jun 16.
DOI: 10.1016/j.ijbiomac.2020.06.132
Abstrakt: Lectins are proteins of non-immunological origin that may play several biological applications, of which we can highlight the anti-inflammatory and antinociceptive activities. In this work, we evaluated the possible effect of orofacial antinociceptive activity of three plant lectins, Dioclea violacea (DVL - Man/Glc-binding), Vatairea macrocarpa (VML - Gal-binding) and PPL (Parkia platycephala - Man/Glc-binding) in adult zebrafish. Acute nociception was induced by menthol (1.2 μM), or capsaicin (4.93 μM) applied into in the upper lip (5.0 μL) of adult wild zebrafish. Zebrafish were pretreated by intraperitoneal injection (20 μL) with vehicle (Control) or lectins (0.025; 0.05 or 0.1 mg/mL) 30 min before induction. The effect of lectins on zebrafish locomotor behavior was evaluated with the open field test. Naive groups (n = 8) were included in all tests. Our results indicate that only PPL presented antinociceptive induced by capsaicin, suggesting the potential clinical application of PPL as inhibitor of orofacial nociception and that this effect may be due to the modulation of TRPV1 channel. In conclusion, lectins that exhibit affinity to the same or different carbohydrates do not necessarily have an antinociceptive effect on the orofacial nociception model, indicating that the glycan carbohydrate binding pattern may be related to the effect on nociception inhibition.
(Copyright © 2020 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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