| Autor: |
Rijkschroeff P; Department of Periodontology, Academic Center for Dentistry Amsterdam (ACTA), University of Amsterdam (UVA) and Vrije Universiteit Amsterdam (VU), Amsterdam, The Netherlands., Schoenmaker T; Department of Periodontology, Academic Center for Dentistry Amsterdam (ACTA), University of Amsterdam (UVA) and Vrije Universiteit Amsterdam (VU), Amsterdam, The Netherlands., Caspers M; Microbiology and System Biology, TNO Earth, Environmental and Life Sciences, Zeist, The Netherlands., Verschuren L; Microbiology and System Biology, TNO Earth, Environmental and Life Sciences, Zeist, The Netherlands., Keijser BJF; Microbiology and System Biology, TNO Earth, Environmental and Life Sciences, Zeist, The Netherlands.; Department of Preventive Dentistry, Academic Center for Dentistry Amsterdam (ACTA), University of Amsterdam and Vrije Universiteit Amsterdam, Amsterdam, The Netherlands.; Top Institute Food and Nutrition, Wageningen, The Netherlands., Nicu EA; Department of Periodontology, Academic Center for Dentistry Amsterdam (ACTA), University of Amsterdam (UVA) and Vrije Universiteit Amsterdam (VU), Amsterdam, The Netherlands.; Opris Dent SRL, Sibiu, Romania., Loos BG; Department of Periodontology, Academic Center for Dentistry Amsterdam (ACTA), University of Amsterdam (UVA) and Vrije Universiteit Amsterdam (VU), Amsterdam, The Netherlands. |
| Abstrakt: |
Oral health and dentistry are essential components of systems medicine, which has received lesser attention in comparison to other medical fields, such as cancer biology. In this context, oral polymorphonuclear neutrophils (oPMNs) play an important role in the maintenance of oral health. To the best of our knowledge, this is the first study to report original observations on the transcriptional responses of oPMNs during experimentally induced gingivitis, by temporarily refraining from regular oral care. Oral rinses were prospectively collected at four different time points for oPMNs isolation from healthy volunteers: day 1 (start of the experimental gingivitis challenge), day 9 (during challenge), day 14 (end of the challenge), and day 21 (postchallenge). Transcriptome of oPMNs was determined by RNA sequencing. Differentially expressed genes (DEGs) were selected at p < 0.01 level, and evaluated for pathway regulation using Ingenuity Pathway Analysis suite. We found four major clusters of DEGs, consisting of 256 initial response DEGs (day 9 only), 221 late response DEGs (day 14 only), 53 persistent responsive DEGs (consistent at day 9 and 14), and 524 DEGs showing responses only in the postchallenge phase (day 21 only). Pathway analysis of the initial and late response DEGs showed involvement in many immune regulatory pathways and PMN function, whereas DEGs at day 21 were associated with epithelial adherence signaling and other miscellaneous related signaling pathways. The results from this pilot study showed that oPMNs mediate oral inflammatory processes, suggesting their immunomodulatory role in oral equilibrium. |
