Comparative study between bone marrow mesenchymal stem cell and their conditioned medium in the treatment of rat model of Parkinsonism.

Autor: Abdelwahab S; Department of Histology and Cell Biology, Faculty of Medicine, Minia University, Minia, Egypt., Elsebay SAG; Department of Histology and Cell Biology, Faculty of Medicine, Ain Shams University, Cairo, Egypt., Fouli Gaber M; Department of Histology and Cell Biology, Faculty of Medicine, Minia University, Minia, Egypt., Abdel-Hafez SMN; Department of Histology and Cell Biology, Faculty of Medicine, Minia University, Minia, Egypt.
Jazyk: angličtina
Zdroj: Journal of cellular physiology [J Cell Physiol] 2021 Jan; Vol. 236 (1), pp. 440-457. Date of Electronic Publication: 2020 Jun 17.
DOI: 10.1002/jcp.29872
Abstrakt: Parkinsonism is one of the most common aging neurodegenerative disorders. This study aims to compare the therapeutic effect of stem cell versus its conditioned medium in the Parkinsonism model. Parkinsonism was induced by daily subcutaneous injection of 0.5 mg/kg of rotenone dissolved in dimethyl sulfoxide for 28 days. Fifty rats were divided randomly into five groups: control, dimethyl sulfoxide, Parkinsonism, stem cell-treated, and conditioned medium-treated groups. Midbrain specimens were obtained for histological, immunohistochemical, and biochemical studies. Lewy bodies were observed in the Parkinsonism group in the dopaminergic neuron and neuropil as well. Almost all of the pathological changes were clearly ameliorated in both stem cell- and conditioned medium-treated groups as confirmed by biochemical, histological, and immunohistochemical (anti-nestin, anti-glial fibrillary acidic protein, and anti-α synuclein) studies. However, the conditioned medium showed more superior therapeutic effect establishing nearly the normal histological architecture of substantia nigra. These results may pave the future for using stem cell-conditioned medium as a more convenient and effective adjuvant therapy in Parkinsonism and other neurodegenerative disorders.
(© 2020 Wiley Periodicals LLC.)
Databáze: MEDLINE
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