| Autor: |
Lindahl LM; Department of Dermatology, Aarhus University Hospital, P.P. Oerumsgade 11, DK-8200 Aarhus N, Denmark. E-mail: lise.lindahl@clin.au.dk., Gluud M, Emmanuel T, Thomsen EA, Hu T, Rittig AH, Celis P, Stolearenco V, Krejsgaard T, Johansen C, Willerslev-Olsen A, Buus TB, Woetmann A, Aagaard L, Geisler C, Litman T, Mikkelsen JG, Odum N, Iversen L |
| Jazyk: |
angličtina |
| Zdroj: |
Acta dermato-venereologica [Acta Derm Venereol] 2020 Sep 30; Vol. 100 (16), pp. adv00270. Date of Electronic Publication: 2020 Sep 30. |
| DOI: |
10.2340/00015555-3574 |
| Abstrakt: |
A prognostic 3-miRNA classifier for early-stage mycosis fungoides has been developed recently, with miR-106b providing the strongest prognostic power. The aim of this study was to investigate the molecular function of miR-106b in mycosis fungoides disease progression. The cellular localization of miR-106b in mycosis fungoides skin biopsies was determined by in situ hybridization. The regulatory role of miR-106b was assessed by transient miR-106b inhibitor/mimic transfection of 2 mycosis fungoides derived cell lines, followed by quantitative real-time PCR (RT-qPCR), western blotting and a proliferation assay. MiR-106b was found to be expressed by dermal T-lymphocytes in mycosis fungoides skin lesions, and miR-106b expression increased with advancing mycosis fungoides stage. Transfection of miR-106b in 2 mycosis fungoides derived cell lines showed that miR-106b represses the tumour suppressors cyclin-dependent kinase inhibitor 1 (p21) and thioredoxin-interacting protein (TXNIP) and promotes mycosis fungoides tumour cell proliferation. In conclusion, these results substantiate that miR-106b has both a functional and prognostic role in progression of mycosis fungoides. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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