| Autor: |
Lochab V; Department of Mechanical & Aerospace Engineering, The Ohio State University, Columbus, Ohio, USA., Jones TH; Department of Mechanical & Aerospace Engineering, The Ohio State University, Columbus, Ohio, USA., Dusane DH; Department of Microbial Infection & Immunity, The Ohio State University, Columbus, Ohio, USA., Peters CW; Department of Microbial Infection & Immunity, The Ohio State University, Columbus, Ohio, USA., Stoodley P; Department of Microbial Infection & Immunity, The Ohio State University, Columbus, Ohio, USA.; Department of Orthopaedics, The Ohio State University, Columbus, Ohio, USA.; National Centre for Advanced Tribology, Mechanical Engineering, University of Southampton, Southampton, UK., Wozniak DJ; Department of Microbial Infection & Immunity, The Ohio State University, Columbus, Ohio, USA., Subramaniam VV; Department of Mechanical & Aerospace Engineering, The Ohio State University, Columbus, Ohio, USA., Prakash S; Department of Mechanical & Aerospace Engineering, The Ohio State University, Columbus, Ohio, USA. prakash.31@osu.edu. |
| Abstrakt: |
Electrochemically generated bactericidal compounds have been shown to eradicate bacterial lawn biofilms through electroceutical treatment. However, the ultrastructure of biofilms exposed to these species has not been studied. Moreover, it is unknown if the efficacy of electroceutical treatment extends to antibiotic-resistant variants that emerge in lawn biofilms after antibiotic treatment. In this report, the efficacy of the in vitro electroceutical treatment of Pseudomonas aeruginosa biofilms is demonstrated both at room temperature and in an incubator, with a ~4 log decrease (p < 0.01) in the biofilm viability observed over the anode at both conditions. The ultrastructure changes in the lawn biofilms imaged using transmission electron microscopy demonstrate significant bacterial cell damage over the anode after 24 h of electroceutical treatment. A mix of both damaged and undamaged cells was observed over the cathode. Finally, both eradication and prevention of the emergence of tobramycin-resistant variants were demonstrated by combining antibiotic treatment with electroceutical treatment on the lawn biofilms. |
