Protective effects of hesperetin on lipopolysaccharide-induced acute lung injury in a rat model.

Autor:	Kaya S; Department of Thoracic Surgery, Tekirdağ Namık Kemal University, Faculty of Medicine, Tekirdağ, Turkey., Albayrak Kaya S; Department of Midwifery, Biruni University, Faculty of Health Sciences, Istanbul, Turkey., Polat E; Department of Histology and Embriology, Tekirdağ Namık Kemal University, Faculty of Medicine, Tekirdağ, Turkey., Fidanol Erboğa Z; Department of Histology and Embriology, Tekirdağ Namık Kemal University, Faculty of Medicine, Tekirdağ, Turkey., Duran Y; Department of General Surgery, Tekirdağ Namık Kemal University, Faculty of Medicine, Tekirdağ, Turkey., Polat FR; Department of General Surgery, Tekirdağ Namık Kemal University, Faculty of Medicine, Tekirdağ, Turkey., Okuyan HM; Department of Medical Services and Techniquies, Mustafa Kemal University, Hatay Vocational School of Health Sciences, Hatay, Turkey., Karaboğa İ; Department of Emergency and Disaster Medicine, Tekirdağ Namık Kemal University, School of Health, Tekirdağ, Turkey.
Jazyk:	angličtina
Zdroj:	Turk gogus kalp damar cerrahisi dergisi [Turk Gogus Kalp Damar Cerrahisi Derg] 2020 Apr 22; Vol. 28 (2), pp. 359-368. Date of Electronic Publication: 2020 Apr 22 (Print Publication: 2019).
DOI:	10.5606/tgkdc.dergisi.2020.18816
Abstrakt:	Background: In this experimental study, we aimed to investigate the effects of hesperetin, a natural flavonoid, on a lipopolysaccharideinduced acute lung injury model in rats. Methods: Between March 2019 and May 2019, a total of 18 adult male Wistar albino rats, weighing approximately 250 to 300 g, were randomly divided into three groups as control, lipopolysaccharide, and lipopolysaccharide + hesperetin groups (n=6 in each group). The wet/dry weight ratio of lung tissue was determined. Histopathological changes were examined using light and scanning electron microscopy. Pulmonary nuclear factor-kappa beta, inducible nitric oxide synthase, and alpha-smooth muscle antigen activity were determined with indirect immunohistochemical methods. Pulmonary apoptosis was detected with the terminal deoxynucleotidyl transferase dUTP nick-end labeling method. Tumor necrosis factor-alpha, interleukin-1 beta, interleukin-6, and interleukin-10 concentrations were measured with enzyme-linked immunosorbent assay. Results: Treatment with hesperetin significantly improved the architecture of lung tissue and reduced the wet/dry weight ratio, nuclear factor-kappa beta, inducible nitric oxide synthase, and alphasmooth muscle antigen expression, pulmonary apoptosis, and levels of proinflammatory cytokines. Conclusion: Our study results suggest that hesperetin has a potent protective effect against lipopolysaccharide-induced acute lung injury in rats via suppression of the proinflammatory cytokine cascade, nuclear factor-kappa beta, signaling pathway activation, and apoptosis. Competing Interests: Conflict of Interest: The authors declared no conflicts of interest with respect to the authorship and/or publication of this article. (Copyright © 2020, Turkish Society of Cardiovascular Surgery.)
Databáze:	MEDLINE
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