Pathodynamics of Circulating Strains of Duck Enteritis Virus: A Step Forward to Understand Its Pathogenesis.

Autor: Sarmah H; Department of Microbiology, College of Veterinary Sciences, Assam Agricultural University, Khanapara, Guwahati, Assam, India 781022., Shah M; Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, India 781039., Pathak M; Department of Pathology, College of Veterinary Sciences, Assam Agricultural University, Khanapara, Guwahati, Assam, India 781022., Barman NN; Department of Microbiology, College of Veterinary Sciences, Assam Agricultural University, Khanapara, Guwahati, Assam, India 781022., Koul M; Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, India 781039., Gupta A; Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, India 781039., Sahariah PJ; Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, India 781039., Neher S; Department of Microbiology, College of Veterinary Sciences, Assam Agricultural University, Khanapara, Guwahati, Assam, India 781022., Das SK; Department of Microbiology, College of Veterinary Sciences, Assam Agricultural University, Khanapara, Guwahati, Assam, India 781022., Gogoi SM; Department of Microbiology, College of Veterinary Sciences, Assam Agricultural University, Khanapara, Guwahati, Assam, India 781022., Kumar S; Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, India 781039, sachinku@iitg.ac.in.
Jazyk: angličtina
Zdroj: Avian diseases [Avian Dis] 2020 Jun; Vol. 64 (2), pp. 166-173.
DOI: 10.1637/0005-2086-64.2.166
Abstrakt: Duck enteritis virus (DEV) causes an acute and contagious infection in duck. The present study was carried out to evaluate the pathogenicity and pathodynamics of DEV isolates from different natural outbreaks in the Assam Province of India. A total of six wild-type isolates of DEV were revived in ducklings to determine its biologic characterization. Postmortem examination of infected ducklings revealed DEV-specific gross lesions in different organs. The presence of DEV was confirmed by its genome amplification and the presence of viral antigens from collected tissue samples by indirect fluorescent antibody test. All the isolates revived in ducklings were further propagated in duck embryo fibroblast cells. Highly virulent and low virulent isolates of DEV were selected for further study based on median duck infectivity dose (DID 50 ) and median tissue culture infectivity dose (TCID 50 ). The highly virulent isolate of DEV had values of 10 2 DID 50 /ml and 10 6.33 TCID 50 /ml, whereas the low virulent strain had titers of 10 DID 50 /ml and 10 4.83 TCID 50 /ml in the cell culture. Our results showed replication of DEV in ducks with the highest and lowest viral titers in the thymus and bursa of Fabricius, respectively. In addition, microscopic analysis revealed necrosis and degeneration of submucosal esophageal glands and glandular epithelium. The study will be useful to understand the organ tropism and pathologic alteration among the virulent DEV isolates.
Databáze: MEDLINE
Externí odkaz: