| Autor: |
Köster DV; Centre for Mechanochemical Cell Biology and Division of Biomedical Sciences, Warwick Medical School, University of Warwick, Coventry, UK. D.Koester@warwick.ac.uk. |
| Jazyk: |
angličtina |
| Zdroj: |
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2020; Vol. 2169, pp. 167-174. |
| DOI: |
10.1007/978-1-0716-0732-9_15 |
| Abstrakt: |
Here, we describe how to extract tethers or lipid membrane nanotubes from the plasma membrane of cells using optical tweezers. This technique allows measuring the force required to hold the membrane tether at a constant length, which is related to the cell membrane tension. Following the evolution of this force during mechanical or chemical perturbations of the cell gives insight about the regulation of cell membrane tension. By pulling very long membrane tethers, one can also probe the membrane reservoir of a cell and a sudden rise in the tether force is usually due to the depletion of excess membranes stored in membrane folds or invaginations. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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