| Autor: |
Podkalicka J; Laboratoire Physico-Chimie Curie, Institut Curie, PSL Research University, Sorbonne Université, CNRS UMR168, Paris, France. joanna.podkalicka@curie.fr.; Institut Curie - Centre de Recherche, PSL Research University, Membrane Mechanics and Dynamics of Intracellular Signaling Laboratory, CNRS UMR3666, INSERM U1143, Paris, France. joanna.podkalicka@curie.fr.; Laboratory of Cytobiochemistry, Faculty of Biotechnology, University of Wrocław, Wrocław, Poland. joanna.podkalicka@curie.fr., Blouin CM; Institut Curie - Centre de Recherche, PSL Research University, Membrane Mechanics and Dynamics of Intracellular Signaling Laboratory, CNRS UMR3666, INSERM U1143, Paris, France. |
| Jazyk: |
angličtina |
| Zdroj: |
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2020; Vol. 2169, pp. 81-88. |
| DOI: |
10.1007/978-1-0716-0732-9_8 |
| Abstrakt: |
Caveolins, major components of small plasma membrane invaginations called caveolae, play a role in signaling, particularly in mechanosignaling. These proteins are known to interact with a variety of effector molecules, including G-protein-coupled receptors, Src family kinases, ion channels, endothelial nitric oxide synthase (eNOS), adenylyl cyclases, protein kinase A (PKA), and mitogen-activated PKs (MAPKs). There is, however, speculation on the relevance of these interactions and the mechanisms by which caveolins may control intracellular signaling. This chapter introduces a method of isolation of giant plasma membrane-derived vesicles (GPMVs), which possess full complexity of membrane they originate from, thus comprising an excellent platform to revisit some of the previously described interactions in a cleaner environment and possibly identifying new binding partners. It is also a powerful technique for studying membrane mechanics, as it was previously used to demonstrate the role of caveolae in mechanoprotection. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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