Profiling lysophosphatidic acid levels in plasma from head and neck cancer patients.

Autor:	Abdul Rahman M; Department of Oral and Craniofacial Sciences, University of Malaya, Kuala Lumpur, Malaysia.; Department of Craniofacial Diagnostics and Biosciences, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia., Mohamad Haron DE; Department of Pharmacology, University of Malaya, Kuala Lumpur, Malaysia., Hollows RJ; Institute of Immunology and Immunotherapy, University of Birmingham, Birmingham, United Kingdom., Abdul Ghani ZDF; Industrial Biotechnology Research Centre (IBRC), SIRIM Berhad, Selangor, Malaysia., Ali Mohd M; Faculty of Medicine and Health Sciences, UCSI University, Kuala Lumpur, Malaysia., Chai WL; Department of Restorative Dentistry, University of Malaya, Kuala Lumpur, Malaysia., Ng CC; Institute of Biological Sciences, University of Malaya, Kuala Lumpur, Malaysia., Lye MS; Department of Community Medicine, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, Malaysia., Karsani SA; Institute of Biological Sciences, University of Malaya, Kuala Lumpur, Malaysia., Yap LF; Department of Oral and Craniofacial Sciences, University of Malaya, Kuala Lumpur, Malaysia., Paterson IC; Department of Oral and Craniofacial Sciences, University of Malaya, Kuala Lumpur, Malaysia.; Oral Cancer Research and Coordinating Centre, University of Malaya, Kuala Lumpur, Malaysia.
Jazyk:	angličtina
Zdroj:	PeerJ [PeerJ] 2020 Jun 05; Vol. 8, pp. e9304. Date of Electronic Publication: 2020 Jun 05 (Print Publication: 2020).
DOI:	10.7717/peerj.9304
Abstrakt:	Head and neck squamous cell carcinoma (HNSCC) represents a significant world health problem, with approximately 600,000 new cases being diagnosed annually. The prognosis for patients with HNSCC is poor and, therefore, the identification of biomarkers for screening, diagnosis and prognostication would be clinically beneficial. A limited number of studies have used lipidomics to profile lipid species in the plasma of cancer patients. However, the profile and levels of lysophosphatidic acid (LPA) species have not been examined in HNSCC. In this study, a targeted lipidomics approach using liquid chromatography triple quadrupole mass spectrometry (LCMS/MS) was used to analyse the concentration of LPA (16:0 LPA, 18:0 LPA, 18:1 LPA, 18:2 LPA and 20:4 LPA) in the plasma of patients with oral squamous cell carcinoma (OSCC) and nasopharyngeal carcinoma (NPC), together with healthy controls. The levels of three LPA species (18:1 LPA, 18:2 LPA and 20:4 LPA) were significantly lower in the plasma of OSCC patients, whilst the concentrations of all five LPA species tested were significantly lower in plasma from NPC patients. Furthermore, the order of abundance of LPA species in plasma was different between the control and cancer groups, with 16:0 LPA, 18:0 LPA levels being more abundant in OSCC and NPC patients. Medium to strong correlations were observed using all pairs of LPA species and a clear separation of the normal and tumour groups was observed using PCA analysis. In summary, the results of this study showed that the levels of several LPA species in the plasma of patients with OSCC and NPC were lower than those from healthy individuals. Understanding these variations may provide novel insights into the role of LPA in these cancers. Competing Interests: The authors declare there are no competing interests. (©2020 Abdul Rahman et al.)
Databáze:	MEDLINE
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