Exploration of Matrix Effects in Laser Ablation Inductively Coupled Plasma Mass Spectrometry Imaging of Cisplatin-Treated Tumors.

Autor:	Greenhalgh CJ; Department of Chemistry, Loughborough University, Loughborough, Leicestershire LE11 3TU, U.K., Karekla E; Leicester Cancer Research Centre, University of Leicester, Robert Kilpatrick Building, Leicester Royal Infirmary, Leicester LE2 7LX, U.K., Miles GJ; Leicester Cancer Research Centre, University of Leicester, Robert Kilpatrick Building, Leicester Royal Infirmary, Leicester LE2 7LX, U.K., Powley IR; Leicester Cancer Research Centre, University of Leicester, Robert Kilpatrick Building, Leicester Royal Infirmary, Leicester LE2 7LX, U.K., Costa C; Ion Beam Centre, University of Surrey, Guildford GU2 7XH, U.K., de Jesus J; Department of Chemistry, University of Surrey, Guildford GU2 7XH, U.K., Bailey MJ; Department of Chemistry, University of Surrey, Guildford GU2 7XH, U.K., Pritchard C; Leicester Cancer Research Centre, University of Leicester, Robert Kilpatrick Building, Leicester Royal Infirmary, Leicester LE2 7LX, U.K., MacFarlane M; MRC Toxicology Unit, Hodgkin Building, Leicester LE1 9HN, U.K., Pringle JH; Leicester Cancer Research Centre, University of Leicester, Robert Kilpatrick Building, Leicester Royal Infirmary, Leicester LE2 7LX, U.K., Managh AJ; Department of Chemistry, Loughborough University, Loughborough, Leicestershire LE11 3TU, U.K.
Jazyk:	angličtina
Zdroj:	Analytical chemistry [Anal Chem] 2020 Jul 21; Vol. 92 (14), pp. 9847-9855. Date of Electronic Publication: 2020 Jul 01.
DOI:	10.1021/acs.analchem.0c01347
Abstrakt:	The use of a low aerosol dispersion ablation chamber within a laser ablation inductively coupled plasma mass spectrometer (LA-ICP-MS) setup allows for high-resolution, high-speed imaging of the distribution of elements within a sample. Here we show how this enhanced capability creates new analytical problems and solutions. We report the distribution of platinum at the cellular level in non-small cell lung cancer (NSCLC) explant models after treatment with clinically relevant doses of cisplatin. This revealed for the first time a correlation between the platinum signal and the presence of carbon deposits within lung tissue. We show how complementary ion beam analysis techniques, particle-induced X-ray emission (PIXE) and elastic backscattering spectrometry (EBS), can be used to explore potential matrix effects in LA-ICP-MS data. For these samples, it was confirmed that the enhancement was unlikely to have resulted from a matrix effect alone. Thus, the presence of carbon deposits within tissue has potential implications for the effective distribution of the cisplatin drug.
Databáze:	MEDLINE
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