Slc26a9 P2ACre : a new CRE driver to regulate gene expression in the otic placode lineage and other FGFR2b-dependent epithelia.

Autor:	Urness LD; Department of Human Genetics, University of Utah, Salt Lake City, UT 84112, USA., Wang X; Department of Human Genetics, University of Utah, Salt Lake City, UT 84112, USA., Li C; Department of Human Genetics, University of Utah, Salt Lake City, UT 84112, USA., Quadros RM; Mouse Genome Engineering Core Facility, Vice Chancellor for Research Office, University of Nebraska Medical Center, Omaha, NE 68198, USA., Harms DW; Mouse Genome Engineering Core Facility, Vice Chancellor for Research Office, University of Nebraska Medical Center, Omaha, NE 68198, USA., Gurumurthy CB; Mouse Genome Engineering Core Facility, Vice Chancellor for Research Office, University of Nebraska Medical Center, Omaha, NE 68198, USA.; Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, NE 68198, USA., Mansour SL; Department of Human Genetics, University of Utah, Salt Lake City, UT 84112, USA suzi.mansour@genetics.utah.edu.
Jazyk:	angličtina
Zdroj:	Development (Cambridge, England) [Development] 2020 Jul 08; Vol. 147 (13). Date of Electronic Publication: 2020 Jul 08.
DOI:	10.1242/dev.191015
Abstrakt:	Pan-otic CRE drivers enable gene regulation throughout the otic placode lineage, comprising the inner ear epithelium and neurons. However, intersection of extra-otic gene-of-interest expression with the CRE lineage can compromise viability and impede auditory analyses. Furthermore, extant pan-otic CREs recombine in auditory and vestibular brain nuclei, making it difficult to ascribe resulting phenotypes solely to the inner ear. We have previously identified Slc26a9 as an otic placode-specific target of the FGFR2b ligands FGF3 and FGF10. We show here that Slc26a9 is otic specific through E10.5, but is not required for hearing. We targeted P2ACre to the Slc26a9 stop codon, generating Slc26a9 P2ACre mice, and observed CRE activity throughout the otic epithelium and neurons, with little activity evident in the brain. Notably, recombination was detected in many FGFR2b ligand-dependent epithelia. We generated Fgf10 and Fgf8 conditional mutants, and activated an FGFR2b ligand trap from E17.5 to P3. In contrast to analogous mice generated with other pan-otic CREs, these were viable. Auditory thresholds were elevated in mutants, and correlated with cochlear epithelial cell losses. Thus, Slc26a9 P2ACre provides a useful complement to existing pan-otic CRE drivers, particularly for postnatal analyses. Competing Interests: Competing interestsThe authors declare no competing or financial interests. (© 2020. Published by The Company of Biologists Ltd.)
Databáze:	MEDLINE
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