Disorders of microcirculation in the mechanism of bisphosphonate osteonecrosis: preliminary study in rats.

Autor: Kosach GA; Resident of maxillo-facial surgeon department, First Pavlov State Medical University of St. Peterburg, L'va Tolstogo str., 6-8, Saint Petersburg, Russian Federation, 197022. Electronic address: German1kosach@gmail.com., Petrosyan AL; First Pavlov State Medical University of St. Peterburg, L'va Tolstogo str., 6-8, Saint Petersburg, Russian Federation, 197022., Yaremenko AI; First Pavlov State Medical University of St. Peterburg, L'va Tolstogo str., 6-8, Saint Petersburg, Russian Federation, 197022., Zubareva AA; ENT, Radiological Department, First Pavlov State Medical University of St. Peterburg, L'va Tolstogo str., 6-8, Saint Petersburg, Russian Federation, 197022., Kutukova SI; Maxillo-facial surgeon department, First Pavlov State Medical University of St. Peterburg, L'va Tolstogo str., 6-8, Saint Petersburg, Russia, 197022; SPB City Clinical Oncology Dispensary, Veteranov prospect, 56, Saint Petersburg, Russian Federation, 198255., Yagmurov OD; Department of Forensic Medicine and Jurisprudence, First Pavlov State Medical University of St. Peterburg, L'va Tolstogo str., 6-8, Saint Petersburg, Russian Federation 197022., Chefu SG; Doctor of biological sciences, Department of Pathophysiology with Clinical Physiology, First Pavlov State Medical University of St. Peterburg, L'va Tolstogo str., 6-8, Saint Petersburg, Russia, 197022; Federal Almazov North-West Medical Research Centre, Akkuratova str., 2, Saint Petersburg, Russian Federation, 197341., Molokova VA; Student of dentistry faculty, First Pavlov State Medical University of St. Peterburg, L'va Tolstogo str., 6-8, Saint Petersburg, Russian Federation, 197022., Ignatova VD; Radiologist, First Pavlov State Medical University of St. Peterburg, L'va Tolstogo str., 6-8, Saint Petersburg, Russian Federation, 197022., Kosach SA; Resident, Pediatric dentistry with a course of orthodontics, First Pavlov State Medical University of St. Peterburg, L'va Tolstogo str., 6-8, Saint Petersburg, Russian Federation, 197022., Vlasov TD; Department of Pathophysiology with Clinical Physiology, First Pavlov State Medical University of St. Peterburg, L'va Tolstogo str., 6-8, Saint Petersburg, Russian Federation, 197022; Federal Almazov North-West Medical Research Centre, Akkuratova str., 2, Saint Petersburg, Russian Federation, 197341.
Jazyk: angličtina
Zdroj: The British journal of oral & maxillofacial surgery [Br J Oral Maxillofac Surg] 2020 Nov; Vol. 58 (9), pp. e38-e44. Date of Electronic Publication: 2020 Jun 12.
DOI: 10.1016/j.bjoms.2020.05.030
Abstrakt: We investigated the possibilities of angioprotection and the reduction of osteonecrosis in rats that had been given bisphosphonates. In our experiment, 27 rats were divided into three groups: Group 1 was injected with saline; Group 2 was given zoledronic acid for six weeks; and Group 3 was given zoledronic acid for six weeks, with added doses of sulodexide after three weeks. After that we constructed a model of how the teeth should be extracted. The velocity of linear blood flow in the periodontal area of an extracted tooth in rats was studied using laser and high-frequency Doppler ultrasound (with the application of the vasoactive substance acetylcholine 3% for 1min). We evaluated changes in the structure of the bony tissues of the head using computed tomography, comparing the control group with the saline group. A rapid reduction in microcirculation was detected during the use of zoledronic acid for six weeks. A smaller reduction in microcirculation was detected after three weeks of treatment with sulodexide and zoledronic acid. There was a reduction in blood flow in the mucous membranes and, to a greater extent, in bony tissue. Zoledronic acid causes significant impairment of the periosteal blood flow to the mucous membranes because of a complex of disorders, which includes both the cellular component (impaired endothelium-dependent vasodilation of the mucous membrane vessels) and by reducing the intensity of microcirculation in the mucous membranes and bony tissues. Sulodexide, however, improves the restoration of blood flow and reduces the severity of osteonecrosis.
(Copyright © 2020 The British Association of Oral and Maxillofacial Surgeons. Published by Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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