Kyasanur Forest disease virus non-mouse animal models: a pilot study.

Autor: Nikiforuk AM; Applied Biosafety Research Program, National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg, MB, R3E 3R2, Canada., Tierny K; Special Pathogens Program, National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg, MB, R3E 3R2, Canada., Cutts TA; Applied Biosafety Research Program, National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg, MB, R3E 3R2, Canada., Kobasa DK; Special Pathogens Program, National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg, MB, R3E 3R2, Canada.; Department of Medical Microbiology, and Infectious Diseases, The University of Manitoba, 745 Bannatyne Avenue, Winnipeg, MB, R3E 0J9, Canada., Theriault SS; Applied Biosafety Research Program, National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg, MB, R3E 3R2, Canada.; Department of Microbiology, The University of Manitoba, 213 Buller Building, Winnipeg, MB, R3T 2N2, Canada., Cook BWM; Applied Biosafety Research Program, National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg, MB, R3E 3R2, Canada. bcook@cytophage.com.; Cytophage Technologies Inc, 26 Henlow Bay, Winnipeg, MB, R3Y 1G4, Canada. bcook@cytophage.com.
Jazyk: angličtina
Zdroj: BMC research notes [BMC Res Notes] 2020 Jun 15; Vol. 13 (1), pp. 291. Date of Electronic Publication: 2020 Jun 15.
DOI: 10.1186/s13104-020-05137-8
Abstrakt: Objectives: Mouse models have delivered variable recapitulation of Kyasanur Forest disease (KFD) pathology and consistently demonstrated neurological involvement which may be a limited feature of human disease. With the purpose of more accurately modelling human disease progression we infected several small-mammalian models: guinea pigs, hamsters and ferrets with a titered infectious dose of Kyasanur Forest disease virus (KFDV). Clinical indicators of disease severity were observed for seventeen days, on day eighteen a visual post-mortem analysis of visceral organs was conducted. Viral load in selected tissues was measured to infer disease signs and the establishment of viral replication.
Data Description: Daily monitoring did not reveal any observable signs of illness; weight loss was minimal across species and gross pathology did not indicate severe viral infection. Tissue specific tropism and establishment of viral infection was monitored by quantitative real-time polymerase chain reaction (qRT-PCR). No viral replication was detected in ferrets (n = 0/3), but was present in the spleen of guinea pigs (n = 3/3) and the brain of hamsters (n = 3/3). Low levels of viral RNA were detected in multiple hamster tissues (kidney, liver, lung and spleen) suggesting the possibility of viral tropism and possible adaptation to the host. No serological tests were performed.
Databáze: MEDLINE
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