Phospholipid distribution in the cytoplasmic membrane of Gram-negative bacteria is highly asymmetric, dynamic, and cell shape-dependent.

Autor:	Bogdanov M; Department of Biochemistry and Molecular Biology, McGovern Medical School at The University of Texas Health Science Center at Houston, TX 77030, USA.; Department of Biochemistry, Biotechnology and Pharmacology, Kazan Federal University, Institute of Fundamental Medicine and Biology, Kazan 420008, Russian Federation., Pyrshev K; Department of Biochemistry and Molecular Biology, McGovern Medical School at The University of Texas Health Science Center at Houston, TX 77030, USA.; Laboratory of the Nanobiotechnology, Department of Neurochemistry, Palladin Institute of Biochemistry of the NAS of Ukraine, 9 Leontovycha Street, Kyiv 01601, Ukraine.; Department of Physics of Biological Systems, Institute of Physics, NAS of Ukraine, 46 Nauky Avenue., Kyiv 03680, Ukraine., Yesylevskyy S; Department of Physics of Biological Systems, Institute of Physics, NAS of Ukraine, 46 Nauky Avenue., Kyiv 03680, Ukraine.; Laboratoire Chrono-Environnement UMR CNRS 6249, Université de Bourgogne Franche-Comté, 16 route de Gray, 25030 Besançon Cedex, France., Ryabichko S; Department of Biochemistry and Molecular Biology, McGovern Medical School at The University of Texas Health Science Center at Houston, TX 77030, USA.; Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, NJ 08540, USA., Boiko V; Department of Biochemistry and Molecular Biology, McGovern Medical School at The University of Texas Health Science Center at Houston, TX 77030, USA.; Department of Spectroscopy of Excited States, Institute of Low Temperature and Structure Research, Polish Academy of Sciences, Okólna 2, Wrocław 50-422, Poland., Ivanchenko P; Department of Biochemistry and Molecular Biology, McGovern Medical School at The University of Texas Health Science Center at Houston, TX 77030, USA.; Department of Chemistry and Interdepartmental Centre Nanostructured Interfaces and Surfaces (NIS), University of Torino, 10125 Torino, Italy., Kiyamova R; Department of Biochemistry, Biotechnology and Pharmacology, Kazan Federal University, Institute of Fundamental Medicine and Biology, Kazan 420008, Russian Federation., Guan Z; Department of Biochemistry, Duke University Medical Center, Durham, NC 27710, USA., Ramseyer C; Laboratoire Chrono-Environnement UMR CNRS 6249, Université de Bourgogne Franche-Comté, 16 route de Gray, 25030 Besançon Cedex, France., Dowhan W; Department of Biochemistry and Molecular Biology, McGovern Medical School at The University of Texas Health Science Center at Houston, TX 77030, USA.
Jazyk:	angličtina
Zdroj:	Science advances [Sci Adv] 2020 Jun 03; Vol. 6 (23), pp. eaaz6333. Date of Electronic Publication: 2020 Jun 03 (Print Publication: 2020).
DOI:	10.1126/sciadv.aaz6333
Abstrakt:	The distribution of phospholipids across the inner membrane (IM) of Gram-negative bacteria is unknown. We demonstrate that the IMs of Escherichia coli and Yersinia pseudotuberculosis are asymmetric, with a 75%/25% (cytoplasmic/periplasmic leaflet) distribution of phosphatidylethanolamine (PE) in rod-shaped cells and an opposite distribution in E. coli filamentous cells. In initially filamentous PE-lacking E. coli cells, nascent PE appears first in the periplasmic leaflet. As the total PE content increases from nearly zero to 75%, cells progressively adopt a rod shape and PE appears in the cytoplasmic leaflet of the IM. The redistribution of PE influences the distribution of the other lipids between the leaflets. This correlates with the tendency of PE and cardiolipin to regulate antagonistically lipid order of the bilayer. The results suggest that PE asymmetry is metabolically controlled to balance temporally the net rates of synthesis and translocation, satisfy envelope growth capacity, and adjust bilayer chemical and physical properties. (Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).)
Databáze:	MEDLINE
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