Lasting Gammaproteobacteria profile changes characterized hematological cancer patients who developed oral mucositis following conditioning therapy.

Autor: Mougeot JC; Department of Oral Medicine, Carolinas Medical Center-Atrium Health, Charlotte, NC, USA., Beckman MF; Department of Oral Medicine, Carolinas Medical Center-Atrium Health, Charlotte, NC, USA., Stevens CB; Department of Oral Medicine, Carolinas Medical Center-Atrium Health, Charlotte, NC, USA., Almon KG; Department of Oral Medicine, Carolinas Medical Center-Atrium Health, Charlotte, NC, USA., Morton DS; Department of Oral Medicine, Carolinas Medical Center-Atrium Health, Charlotte, NC, USA., Von Bültzingslöwen I; Institute of Odontology, Sahlgrenska Academy at the University of Gothenburg, Gothenburg, Sweden., Brennan MT; Department of Oral Medicine, Carolinas Medical Center-Atrium Health, Charlotte, NC, USA., Mougeot FB; Department of Oral Medicine, Carolinas Medical Center-Atrium Health, Charlotte, NC, USA.
Jazyk: angličtina
Zdroj: Journal of oral microbiology [J Oral Microbiol] 2020 May 13; Vol. 12 (1), pp. 1761135. Date of Electronic Publication: 2020 May 13 (Print Publication: 2020).
DOI: 10.1080/20002297.2020.1761135
Abstrakt: Background: Oral mucositis (OM) is a common side effect of conditioning therapy implemented before hematopoietic stem cell transplantation (HSCT). The role of oral microbiome in OM is not fully elucidated. Objective: To determine oral microbiome profile changes post-conditioning in HSCT patients who developed moderate OM, or mild to no OM. Design: Patient groups were: Muc0-1 with OM-score = 0-1 (43 paired samples) and Muc2 with WHO OM-score = 2 (36 paired samples). Bacterial DNA was isolated from oral samples (saliva, swabs of buccal mucosa, tongue, and supragingival plaque) at pre-conditioning (T 0 ), post-conditioning mucositis onset (T Muc ), and one-year post-conditioning (T Year ). 16S-rRNA gene next-generation sequencing was used to determine the relative abundance (RA) of >700 oral species. Alpha -diversity, beta -diversity and linear discriminant analyses (LDA) were performed Muc2 versus Muc0-1. Results: Muc2 oral microbiome alpha - and beta -diversity differed between T 0 and T Muc . Muc2 alpha -diversity and Muc0-1 beta- diversity did not differ between T 0 and T Year . T 0 to T Muc LDA scores were significant in Muc2 for Gammaproteobacteria . For Muc2 patients, the average RA decreased for Haemophilus parainfluenza , a species known as mucosal surfaces protector, but increased for Escherichia-Shigella genera. Conclusions: Post-conditioning OM might contribute to long-term oral microbiome changes affecting Gammaproteobacteria , in HSCT patients.
(© 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.)
Databáze: MEDLINE
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