| Autor: |
Budde D; Sartorius Stedim Biotech GmbH, August-Spindler-Str. 11, 37079, Goettingen, Germany. dana.budde@sartorius.com.; Universität Bielefeld, Universitätsstraße 25, 33615, Bielefeld, Germany. dana.budde@sartorius.com., Maier TV; Sartorius Stedim Biotech GmbH, August-Spindler-Str. 11, 37079, Goettingen, Germany. tanja.maier@sartorius.com., Jurkiewicz E; Sartorius Stedim Biotech GmbH, August-Spindler-Str. 11, 37079, Goettingen, Germany., Pahl I; Sartorius Stedim Biotech GmbH, August-Spindler-Str. 11, 37079, Goettingen, Germany., Hauk A; Sartorius Stedim Biotech GmbH, August-Spindler-Str. 11, 37079, Goettingen, Germany., Täuscher E; Technische Universität Ilmenau, Weimarer Straße 25, 98693, Ilmenau, Germany., Görls H; Jena University, Lessingstraße 8, 07743, Jena, Germany., Noll T; Universität Bielefeld, Universitätsstraße 25, 33615, Bielefeld, Germany., Menzel R; Sartorius Stedim Biotech GmbH, August-Spindler-Str. 11, 37079, Goettingen, Germany. |
| Abstrakt: |
The inhibiting effect of the secondary phosphite antioxidant degradation product bis(2,4-di-tert-butylphenyl)phosphate (bDtBPP) on cell growth is well-known. The present study describes structurally related compounds which are likely to be formed from similar widely used phosphite antioxidants used in materials for the manufacturing of single-use (SU) equipment. Two potential candidates of such compounds-3,3',5,5'-tetra-tert-butyl-2,2'-dihydroxybiphenylphosphate (TtBBP) and bis(p-nonylphenyl)phosphate (bNPP)-were identified by chromatography and mass spectrometry followed by synthesis and X-ray structure elucidation. Additionally, the formation of TtBBP was confirmed in an analytical degradation study and its migration from SU bioprocessing material was estimated. The cytotoxicity evaluation by means of cell culture spiking experiments and flow cytometry analysis revealed that' even if cell growth was inhibited by all the compounds to some extent, bDtBPP showed the most severe effect and stoods out from the other two degradants investigated. Graphical abstract. |
