Met-Flow, a strategy for single-cell metabolic analysis highlights dynamic changes in immune subpopulations.

Autor: Ahl PJ; Institute of Molecular and Cell Biology, Agency for Science, Technology and Research, Singapore, 138673, Singapore.; Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, 117545, Singapore., Hopkins RA; Institute of Molecular and Cell Biology, Agency for Science, Technology and Research, Singapore, 138673, Singapore.; Tessa Therapeutics Pte Ltd, Institute of Molecular and Cell Biology, Agency for Science, Technology and Research, Singapore, 138673, Singapore., Xiang WW; Institute of Molecular and Cell Biology, Agency for Science, Technology and Research, Singapore, 138673, Singapore.; Tessa Therapeutics Pte Ltd, Institute of Molecular and Cell Biology, Agency for Science, Technology and Research, Singapore, 138673, Singapore., Au B; Institute of Molecular and Cell Biology, Agency for Science, Technology and Research, Singapore, 138673, Singapore., Kaliaperumal N; Institute of Molecular and Cell Biology, Agency for Science, Technology and Research, Singapore, 138673, Singapore., Fairhurst AM; Institute of Molecular and Cell Biology, Agency for Science, Technology and Research, Singapore, 138673, Singapore., Connolly JE; Institute of Molecular and Cell Biology, Agency for Science, Technology and Research, Singapore, 138673, Singapore. jeconnolly@imcb.a-star.edu.sg.; Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, 117545, Singapore. jeconnolly@imcb.a-star.edu.sg.; Institute of Biomedical Studies, Baylor University, Waco, TX, 76712, USA. jeconnolly@imcb.a-star.edu.sg.
Jazyk: angličtina
Zdroj: Communications biology [Commun Biol] 2020 Jun 12; Vol. 3 (1), pp. 305. Date of Electronic Publication: 2020 Jun 12.
DOI: 10.1038/s42003-020-1027-9
Abstrakt: A complex interaction of anabolic and catabolic metabolism underpins the ability of leukocytes to mount an immune response. Their capacity to respond to changing environments by metabolic reprogramming is crucial to effector function. However, current methods lack the ability to interrogate this network of metabolic pathways at single-cell level within a heterogeneous population. We present Met-Flow, a flow cytometry-based method capturing the metabolic state of immune cells by targeting key proteins and rate-limiting enzymes across multiple pathways. We demonstrate the ability to simultaneously measure divergent metabolic profiles and dynamic remodeling in human peripheral blood mononuclear cells. Using Met-Flow, we discovered that glucose restriction and metabolic remodeling drive the expansion of an inflammatory central memory T cell subset. This method captures the complex metabolic state of any cell as it relates to phenotype and function, leading to a greater understanding of the role of metabolic heterogeneity in immune responses.
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje