| Autor: |
Wei GQ; Shandong Institute of Pomology, Tai'an 271000, Shandong, China., Tao JH; Shandong Institute of Pomology, Tai'an 271000, Shandong, China., Fu QJ; Shandong Institute of Pomology, Tai'an 271000, Shandong, China., Hou S; Shandong Institute of Pomology, Tai'an 271000, Shandong, China., Yang XH; Shandong Institute of Pomology, Tai'an 271000, Shandong, China., Sui SG; Shandong Institute of Pomology, Tai'an 271000, Shandong, China., Yu XM; Shandong Institute of Pomology, Tai'an 271000, Shandong, China., Sun YG; Shandong Institute of Pomology, Tai'an 271000, Shandong, China. |
| Abstrakt: |
To investigate the effects of H 2 S on mitochondrial functions under low temperature stress, we analyzed the effects of 0.05 mmol·L -1 NaHS and 15 μmmol·L -1 HT (hypotaurine and H 2 S scavenger) on mitochondria antioxidant enzyme activities and mitochondrial permeability transition pore, mitochondrial membrane fluidity, mitochondrial membrane potential, Cyt c/a ratio and H + -ATPase activity in sweet cherry stigma and ovary with sweet cherry variety Zaodaguo under -2 ℃ low temperature stress. The results showed that low temperature stress increased the concentrations of mitochondrial H 2 O 2 and MDA, enhanced the mitochondrial membrane permeability, but decreased the mitochondrial membrane fluidity, membrane potential, Cyt c/a and H + -ATPase acti-vity. Application of NaHS at 0.05 mmol·L -1 could effectively reduce the concentrations of H 2 O 2 and MDA, and keep higher activities of SOD, POD and CAT of mitochondrial for longer time. Furthermore, application of 0.05 mmol·L -1 NaHS could decrease mitochondrial membrane permeability while increase mitochondrial membrane fluidity, membrane potential, Cyt c/a and H + -ATPase activity in stigma and ovary under low temperature stress. The effects of NaHS were completely offset by HT addition. The results suggested that exogenous H 2 S could alleviate the oxidative damage on stigma and ovary stress through decreasing H 2 O 2 accumulation, regulating mitochondria antioxidant system, increasing H + -ATPase activity, and mitigating mitochondria function under low temperature. |
