Full-field strain of regenerated bone tissue in a femoral fracture model.

Autor: Karali A; Zeiss Global Centre, School of Mechanical and Design Engineering, University of Portsmouth, Portsmouth, UK., Kao AP; Zeiss Global Centre, School of Mechanical and Design Engineering, University of Portsmouth, Portsmouth, UK., Meeson R; Royal Veterinary College, Hatfield, Hertfordshire, UK., Roldo M; School of Pharmacy and Biomedical Sciences, University of Portsmouth, Portsmouth, UK., Blunn GW; School of Pharmacy and Biomedical Sciences, University of Portsmouth, Portsmouth, UK., Tozzi G; Zeiss Global Centre, School of Mechanical and Design Engineering, University of Portsmouth, Portsmouth, UK.
Jazyk: angličtina
Zdroj: Journal of microscopy [J Microsc] 2022 Mar; Vol. 285 (3), pp. 156-166. Date of Electronic Publication: 2020 Jun 24.
DOI: 10.1111/jmi.12937
Abstrakt: The mechanical behaviour of regenerated bone tissue during fracture healing is key in determining its ability to withstand physiological loads. However, the strain distribution in the newly formed tissue and how this influences the way a fracture heals it is still unclear. X-ray Computed Tomography (XCT) has been extensively used to assess the progress of mineralised tissues in regeneration and when combined with in situ mechanics and digital volume correlation (DVC) has been proven a powerful tool to understand the mechanical behaviour and full-field three-dimensional (3D) strain distribution in bone. The purpose of this study is therefore to use in situ XCT mechanics and DVC to investigate the strain distribution and load-bearing capacity in a regenerating fracture in the diaphyseal bone, using a rodent femoral fracture model stabilised by external fixation. Rat femurs with 1 mm and 2 mm osteotomy gaps were tested under in situ XCT step-wise compression in the apparent elastic region. High strain was present in the newly formed bone (ε p1 and ε p3 reaching 29 000 µε and -43 000 µε, respectively), with a wide variation and inhomogeneity of the 3D strain distribution in the regenerating tissues of the fracture gap, which is directly related to the presence of unmineralised tissue observed in histological images. The outcomes of this study will contribute in understanding natural regenerative ability of bone and its mechanical behaviour under loading.
(© 2020 The Authors. Journal of Microscopy published by John Wiley & Sons Ltd on behalf of Royal Microscopical Society.)
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje