Isolation, characterization, and hepatoprotective properties of betulinic acid and ricinine from Tetracarpidium conophorum seeds (Euphorbiaceae).

Autor: Oriakhi K; Department of Medical Biochemistry, School of Basic Medical Sciences, University of Benin, Benin City, Nigeria.; International Center for Chemical and Biological Sciences, University of Karachi, Karachi, Pakistan., Uadia PO; Department of Biochemistry, Faculty of Life Sciences, University of Benin, Benin City, Nigeria., Shaheen F; International Center for Chemical and Biological Sciences, University of Karachi, Karachi, Pakistan., Jahan H; International Center for Chemical and Biological Sciences, University of Karachi, Karachi, Pakistan., Ibeji CU; Physical Chemistry Division, Pure and Industrial Chemistry, University of Nigeria, Nsukka, Nigeria., Iqbal CM; International Center for Chemical and Biological Sciences, University of Karachi, Karachi, Pakistan.
Jazyk: angličtina
Zdroj: Journal of food biochemistry [J Food Biochem] 2021 Mar; Vol. 45 (3), pp. e13288. Date of Electronic Publication: 2020 Jun 11.
DOI: 10.1111/jfbc.13288
Abstrakt: The present study is to isolate and characterize betulinic acid and ricinine from T. conophorum seeds. Phytochemical investigation on hexane fraction of T. conophorum seeds led to the isolation of two compounds, Betulinic acid (1), and Ricinine (2). Betulinic acid and ricinine were screened against HepG2 cells and tested in vivo in CCl 4 -induced experimental rats model. Results from this study showed that the compounds had hepatoprotective and cytotoxic activities. It was observed that betulinic acid inhibited HepG2 cell with percentage inhibition of 54% compared with standard doxorubicin (64%), while ricinine was inactive against HepG2 cell lines. Furthermore, molecular docking was carried out on betulinic acids and ricinine, with binding energies of -11.2 kcal/mol and -5.4 kcal/mol, respectively, indicating strong binding sites and interactions with Hepatitis B Virus DNA polymerase. Therefore, findings from this study suggest that betulinic acid possess cytotoxic and hepatoprotective properties, while ricinine exhibited hepatoprotection in CCl 4 -induced liver damage. PRACTICAL APPLICATIONS: Medicinal plants contain unrestricted ability to make compounds that intrigue researchers in the quest for novel phyto-therapeutic drugs. The continuous exploration of new compounds in the medicinal plant is an auspicious strategy for the prevention of diseases. Therefore, the purpose of this research is to evaluate the cytotoxic and hepatoprotective compounds (betulinic acid and ricinine) isolated from T. conophorum seeds.
(© 2020 Wiley Periodicals LLC.)
Databáze: MEDLINE
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