Salt-Mediated Nanopore Detection of ADAM-17.
| Autor: | Chen X; Department of Chemistry, Illinois Institute of Technology, Chicago, IL, 60616, USA., Zhang Y; Department of Chemistry, Illinois Institute of Technology, Chicago, IL, 60616, USA., Roozbahani GM; Department of Chemistry, Illinois Institute of Technology, Chicago, IL, 60616, USA., Guan X; Department of Chemistry, Illinois Institute of Technology, Chicago, IL, 60616, USA. |
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| Jazyk: | angličtina |
| Zdroj: | ACS applied bio materials [ACS Appl Bio Mater] 2019 Jan 22; Vol. 2 (1), pp. 504-509. Date of Electronic Publication: 2018 Dec 24. |
| DOI: | 10.1021/acsabm.8b00689 |
| Abstrakt: | ADAM-17 (a disintegrin and metalloproteinase 17) plays an important role in various physiological and pathophysiological processes. Overexpression/underexpression of ADAM-17 could lead to various diseases. In this work, by taking advantage of ionic strength and salt gradient, and monitoring the cleavage of a substrate peptide by ADAM-17 in a nanopore, we developed a label-free sensor for the rapid detection of ADAM-17. The sensor was highly sensitive and selective: picomolar concentrations of ADAM-17 could be detected within minutes, while structure similar proteases such as ADAM-9 and MMP-9 did not interfere with its detection. Our developed nanopore sensing strategy should find useful applications in the development of nanopore sensors for other proteases of biological, pharmaceutical, and medical importance. Competing Interests: The authors declare no competing financial interests. |
| Databáze: | MEDLINE |
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