| Autor: |
Ribeiro THC; Laboratory of Molecular Plant Physiology, Department of Biology, Federal University of Lavras, Lavras, Brazil., Fernandes-Brum CN; Laboratory of Molecular Plant Physiology, Department of Biology, Federal University of Lavras, Lavras, Brazil., de Souza CR; Technological Center of Grape and Wine Research, Agronomical Research Institute of Minas Gerais, Caldas, Brazil., Dias FAN; Technological Center of Grape and Wine Research, Agronomical Research Institute of Minas Gerais, Caldas, Brazil., Almeida-Junior O; Coordination of Integral Technical Assistance of São Paulo, São Paulo, Brazil., Regina MA; Technological Center of Grape and Wine Research, Agronomical Research Institute of Minas Gerais, Caldas, Brazil., de Oliveira KKP; Laboratory of Molecular Plant Physiology, Department of Biology, Federal University of Lavras, Lavras, Brazil., Dos Reis GL; Laboratory of Molecular Plant Physiology, Department of Biology, Federal University of Lavras, Lavras, Brazil., Oliveira LM; Department of Phytopathology, Federal University of Lavras, Lavras, Brazil., Fernandes FP; Technological Center of Grape and Wine Research, Agronomical Research Institute of Minas Gerais, Caldas, Brazil., Torregrosa L; Montpellier SupAgro, Montpellier, France., de Souza JT; Department of Phytopathology, Federal University of Lavras, Lavras, Brazil., Chalfun-Junior A; Laboratory of Molecular Plant Physiology, Department of Biology, Federal University of Lavras, Lavras, Brazil. chalfunjunior@ufla.br. |
| Abstrakt: |
Bud necrosis (BN) is a common disorder that affects Vitis vinifera L. and reduces its potential yield. To minimize the losses caused by BN, the double pruning management was applied in Brazilian Southeast vineyards. In this management strategy plants are pruned at the winter to promote a vegetative cycle and then, at summer, to promote the reproductive cycle at optimal environmental conditions. To investigate the relationship of BN and the double pruning management RNA-seq libraries were sequenced from healthy and necrotic tissues at four different stages of the year. The comparison of differentially expressed genes in necrotic and non-necrotic tissues showed an enhanced expression of genes related to cell death possibly induced by endophytic microorganisms in the necrotic tissues. The de novo assembly, characterization and quantification of transcripts within the RNA-seq libraries showed that genes from the endophytic fungus Alternaria alternata, responsible for the production of toxic compounds were highly expressed under BN. Here we propose a model in which unfavorable conditions and reduced carbohydrate levels in buds can promote the switch from a biotrophic lifestyle to a necrotrophic lifestyle in the endophytic fungi, which seems to be involved in the development of BN. |
