Effects of butyl toluidine blue photosensitizer on antimicrobial photodynamic therapy for experimental periodontitis treatment in rats.

Autor: Nuernberg MAA; Department of Diagnostic and Surgery, Periodontics Division, School of Dentistry, São Paulo State University (UNESP), Araçatuba, São Paulo, Brazil. Electronic address: marta.nuernberg@hotmail.com., Wainwright M; School of Pharmacy and Biomolecular Sciences, Liverpool John Moores University, Liverpool, United Kingdom. Electronic address: mark_wainwright@hotmail.com., Miessi DMJ; Department of Diagnostic and Surgery, Periodontics Division, School of Dentistry, São Paulo State University (UNESP), Araçatuba, São Paulo, Brazil. Electronic address: danijanjacomo@hotmail.com., Scalet V; Department of Diagnostic and Surgery, Periodontics Division, School of Dentistry, São Paulo State University (UNESP), Araçatuba, São Paulo, Brazil. Electronic address: vitorscalet@hotmail.com., Olivo MB; Department of Diagnostic and Surgery, Periodontics Division, School of Dentistry, São Paulo State University (UNESP), Araçatuba, São Paulo, Brazil. Electronic address: marianebocalon.56@hotmail.com., Ervolino E; Department of Basic Sciences, School of Dentistry, São Paulo State University (UNESP), Araçatuba, São Paulo, Brazil. Electronic address: e.ervolino@unesp.br., Garcia VG; Department of Diagnostic and Surgery, Periodontics Division, School of Dentistry, São Paulo State University (UNESP), Araçatuba, São Paulo, Brazil; Latin American Institute of Dental Research and Education (ILAPEO), Curitiba, PR, Brazil. Electronic address: vg.garcia@uol.com.br., Theodoro LH; Department of Diagnostic and Surgery, Periodontics Division, School of Dentistry, São Paulo State University (UNESP), Araçatuba, São Paulo, Brazil. Electronic address: leticia.theodoro@unesp.br.
Jazyk: angličtina
Zdroj: Photodiagnosis and photodynamic therapy [Photodiagnosis Photodyn Ther] 2020 Sep; Vol. 31, pp. 101868. Date of Electronic Publication: 2020 Jun 08.
DOI: 10.1016/j.pdpdt.2020.101868
Abstrakt: Aim: This study evaluated three concentrations of butyl toluidine blue (BuTB) for antimicrobial photodynamic therapy (aPDT) in experimental periodontitis (EP) in rats.
Material and Methods: EP was ligature-induced at the first mandibular molar in 105 rats. Ligature was removed after 7 days and animals were distributed into the following treatments: SRP, scaling and root planing (SRP) plus saline solution; BuTB-0.1, SRP plus BuTB at 0.1 mg/mL; aPDT-0.1, SRP plus BuTB at 0.1 mg/mL and InGaAlP diode laser (DL) irradiation; BuTB-0.5, SRP plus BuTB at 0.5 mg/mL; aPDT-0.5, SRP plus BuTB at 0.5 mg/mL and DL irradiation; BuTB-2.0, SRP plus BuTB at 2 mg/mL; aPDT-2.0, SRP plus BuTB at 2 mg/mL and DL irradiation. Five animals from each group were submitted to euthanasia at 7, 15 and 30 days post-treatment. The furcation area was submitted to histological, histometric and immunohistochemical (TGF-ß1, OCN and TRAP) analyses.
Results: aPDT-0.5 group presented a better tissue remodeling in all periods, resolution of the inflammatory response and bone neoformation areas at 30 days. aPDT-0.5 also resulted in higher immunolabeling patterns of TGF-ß1 at all periods (p < 0.05) and of OCN at 30 days (p < 0.05).
Conclusion: aPDT-0.5 showed the best benefits for inflammatory response and periodontal repair process.
(Copyright © 2020 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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