Competitive Growth Assay of Mutagenized Chlamydomonas reinhardtii Compatible With the International Space Station Veggie Plant Growth Chamber.

Autor:	Zhang J; Horticultural Sciences Department, University of Florida, Gainesville, FL, United States., Müller BSF; Horticultural Sciences Department, University of Florida, Gainesville, FL, United States., Tyre KN; Center for the Advancement of Science in Space, Melbourne, FL, United States., Hersh HL; Horticultural Sciences Department, University of Florida, Gainesville, FL, United States., Bai F; Horticultural Sciences Department, University of Florida, Gainesville, FL, United States., Hu Y; Horticultural Sciences Department, University of Florida, Gainesville, FL, United States., Resende MFR Jr; Horticultural Sciences Department, University of Florida, Gainesville, FL, United States., Rathinasabapathi B; Horticultural Sciences Department, University of Florida, Gainesville, FL, United States., Settles AM; Horticultural Sciences Department, University of Florida, Gainesville, FL, United States.
Jazyk:	angličtina
Zdroj:	Frontiers in plant science [Front Plant Sci] 2020 May 25; Vol. 11, pp. 631. Date of Electronic Publication: 2020 May 25 (Print Publication: 2020).
DOI:	10.3389/fpls.2020.00631
Abstrakt:	A biological life support system for spaceflight would capture carbon dioxide waste produced by living and working in space to generate useful organic compounds. Photosynthesis is the primary mechanism to fix carbon into organic molecules. Microalgae are highly efficient at converting light, water, and carbon dioxide into biomass, particularly under limiting, artificial light conditions that are a necessity in space photosynthetic production. Although there is great promise in developing algae for chemical or food production in space, most spaceflight algae growth studies have been conducted on solid agar-media to avoid handling liquids in microgravity. Here we report that breathable plastic tissue culture bags can support robust growth of Chlamydomonas reinhardtii in the Veggie plant growth chamber, which is used on the International Space Station (ISS) to grow terrestrial plants. Live cultures can be stored for at least 1 month in the bags at room temperature. The gene set required for growth in these photobioreactors was tested using a competitive growth assay with mutations induced by short-wave ultraviolet light (UVC) mutagenesis in either wild-type (CC-5082) or cw15 mutant (CC-1883) strains at the start of the assay. Genome sequencing identified UVC-induced mutations, which were enriched for transversions and non-synonymous mutations relative to natural variants among laboratory strains. Genes with mutations indicating positive selection were enriched for information processing genes related to DNA repair, RNA processing, translation, cytoskeletal motors, kinases, and ABC transporters. These data suggest that modification of DNA repair, signal transduction, and metabolite transport may be needed to improve growth rates in this spaceflight production system. (Copyright © 2020 Zhang, Müller, Tyre, Hersh, Bai, Hu, Resende, Rathinasabapathi and Settles.)
Databáze:	MEDLINE
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