An atypical clinicopathological manifestation of fowlpox virus associated with reticuloendotheliosis virus in commercial laying hen flocks in Brazil.

Autor: Chacón RD; Department of Pathology, School of Veterinary Medicine, University of São Paulo, São Paulo, Brazil.; Inter-units Program in Biotechnology, University of São Paulo, São Paulo, Brazil., Astolfi-Ferreira CS; Department of Pathology, School of Veterinary Medicine, University of São Paulo, São Paulo, Brazil., De la Torre DI; Department of Pathology, School of Veterinary Medicine, University of São Paulo, São Paulo, Brazil., de Sá LRM; Department of Pathology, School of Veterinary Medicine, University of São Paulo, São Paulo, Brazil., Piantino Ferreira AJ; Department of Pathology, School of Veterinary Medicine, University of São Paulo, São Paulo, Brazil.
Jazyk: angličtina
Zdroj: Transboundary and emerging diseases [Transbound Emerg Dis] 2020 Nov; Vol. 67 (6), pp. 2923-2935. Date of Electronic Publication: 2020 Jun 24.
DOI: 10.1111/tbed.13668
Abstrakt: Fowlpox (FP) is a common epitheliotropic disease in chickens that is usually controlled by live attenuated vaccines. However, there have been some reports of outbreaks of FP in recent years, even in vaccinated flocks, presenting as atypical lesions and feathering abnormalities in chickens. These findings can be associated with fowlpox virus (FPV) with the reticuloendotheliosis virus (REV) integrated into its genome. In the present study, outbreaks of atypical FP were explored in vaccinated commercial laying hen flocks to determine the nature of the causative agent by histopathologic and molecular approaches. FPV and REV were detected and classified into subclade A1 of the genus Avipoxvirus and subtype 3 of REV (REV3), respectively. Additionally, heterogeneous populations of FPV with partial (containing only a remnant long terminal repeat-LTR) or total (all functional genes) integration of REV were identified by heterologous PCRs and detected considering reference integration sites. These results indicate the mechanism of chimeric genome FPV-REV associated with outbreaks and atypical clinicopathological manifestations in commercial laying hens for the first time in Brazil and in South America. In addition, this study demonstrates the emergence of REV integrated in the FPV genome in Brazilian chicken flocks.
(© 2020 Blackwell Verlag GmbH.)
Databáze: MEDLINE
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