Cell jamming, stratification and p63 expression in cultivated human corneal epithelial cell sheets.

Autor: Baba K; Department of Ophthalmology, Graduate School of Medicine, Osaka University, 2-2  Yamadaoka, Suita, Osaka, 565-0871, Japan., Sasaki K; Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-2 Yamadaoka, Suita, Osaka, 565-0871, Japan.; Global Center for Medical Engineering and Informatics, Osaka University, 2-2 Yamadaoka, Suita, Osaka, 565-0871, Japan., Morita M; Department of Ophthalmology, Graduate School of Medicine, Osaka University, 2-2  Yamadaoka, Suita, Osaka, 565-0871, Japan., Tanaka T; Japan Tissue Engineering Co., Ltd, 6-209-1 Miyakitadori, Gamagori, Aichi, 443-0022, Japan., Teranishi Y; Japan Tissue Engineering Co., Ltd, 6-209-1 Miyakitadori, Gamagori, Aichi, 443-0022, Japan., Ogasawara T; Japan Tissue Engineering Co., Ltd, 6-209-1 Miyakitadori, Gamagori, Aichi, 443-0022, Japan., Oie Y; Department of Ophthalmology, Graduate School of Medicine, Osaka University, 2-2  Yamadaoka, Suita, Osaka, 565-0871, Japan., Kusumi I; Department of Ophthalmology, Graduate School of Medicine, Osaka University, 2-2  Yamadaoka, Suita, Osaka, 565-0871, Japan., Inoie M; Japan Tissue Engineering Co., Ltd, 6-209-1 Miyakitadori, Gamagori, Aichi, 443-0022, Japan., Hata KI; Japan Tissue Engineering Co., Ltd, 6-209-1 Miyakitadori, Gamagori, Aichi, 443-0022, Japan., Quantock AJ; Structural Biophysics Group, School of Optometry and Vision Sciences, Cardiff University, Maindy Road, Cardiff, Wales, CF24 4HQ, United Kingdom., Kino-Oka M; Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-2 Yamadaoka, Suita, Osaka, 565-0871, Japan., Nishida K; Department of Ophthalmology, Graduate School of Medicine, Osaka University, 2-2  Yamadaoka, Suita, Osaka, 565-0871, Japan. knishida@ophthal.med.osaka-u.ac.jp.; Integrated Frontier Research for Medical Science Division, Institute for Open and Transdisciplinary Research Initiatives (OTRI), Osaka University, 2-2 Yamadaoka, Suita, Osaka, 565-0871, Japan. knishida@ophthal.med.osaka-u.ac.jp.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2020 Jun 09; Vol. 10 (1), pp. 9282. Date of Electronic Publication: 2020 Jun 09.
DOI: 10.1038/s41598-020-64394-6
Abstrakt: Corneal limbal epithelial stem cell transplantation using cultivated human corneal epithelial cell sheets has been used successfully to treat limbal stem cell deficiencies. Here we report an investigation into the quality of cultivated human corneal epithelial cell sheets using time-lapse imaging of the cell culture process every 20 minutes over 14 days to ascertain the level of cell jamming, a phenomenon in which cells become smaller, more rounded and less actively expansive. In parallel, we also assessed the expression of p63, an important corneal epithelial stem cell marker. The occurrence of cell jamming was variable and transient, but was invariably associated with a thickening and stratification of the cell sheet. p63 was present in all expanding cell sheets in the first 9 days of culture, but it's presence did not always correlate with stratification of the cell sheet. Nor did p63 expression necessarily persist in stratified cell sheets. An assessment of cell jamming, therefore, can shed significant light on the quality and regenerative potential of cultivated human corneal epithelial cell sheets.
Databáze: MEDLINE
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