A Fluorescence in Situ Hybridization (FISH) Test for Diagnosing Babesiosis.

Autor: Shah JS; ID-FISH Technology Inc., 556 Gibraltar Drive, Milpitas, CA 95035, USA.; IGenex Inc., 556 Gibraltar Drive, Milpitas, CA 95035, USA., Mark O; IGenex Inc., 556 Gibraltar Drive, Milpitas, CA 95035, USA., Caoili E; IGenex Inc., 556 Gibraltar Drive, Milpitas, CA 95035, USA., Poruri A; IGenex Inc., 556 Gibraltar Drive, Milpitas, CA 95035, USA., Horowitz RI; Hudson Valley Healing Arts Center, New York, NY 12538, USA.; Member, HHS Subcommittee on Babesia and Tick-Borne Pathogens, US Department of Health and Human Services, Washington, DC 20201, USA., Ashbaugh AD; College of Medicine, University of Cincinnati, Cincinnati, OH 45221, USA., Ramasamy R; ID-FISH Technology Inc., 556 Gibraltar Drive, Milpitas, CA 95035, USA.
Jazyk: angličtina
Zdroj: Diagnostics (Basel, Switzerland) [Diagnostics (Basel)] 2020 Jun 06; Vol. 10 (6). Date of Electronic Publication: 2020 Jun 06.
DOI: 10.3390/diagnostics10060377
Abstrakt: Apicomplexan parasites of the genus Babesia cause babesiosis in humans and animals. The microscopic examination of stained blood smears, detection of serum antibodies by immunoassays, and PCR-based identification of parasite nucleic acid in blood are common laboratory methods for diagnosing babesiosis. The present study evaluated a commercially available Babesia genus-specific fluorescence in situ hybridization (FISH) test for detecting Babesia parasites in blood smears. The FISH test detected Babesia duncani and Babesia microti , two common species that cause human infections in the USA, and other Babesia species of human and veterinary importance in less than two hours. The Babesia genus-specific FISH test supplements other existing laboratory methods for diagnosing babesiosis and may be particularly useful in resource-limited laboratories.
Competing Interests: J.S.S. owns stock and is chief executive of IGeneX and scientific director of IDFISH; R.R.is an affiliate of IDFISH; O.M., E.C., and A.P. are employees at IGeneX and IDFISH.
Databáze: MEDLINE
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