Cell-free long non-coding RNAs (LY86-AS1 & HCG27_201and GAS5) as biomarkers for pre-diabetes and type 2 DM in Egypt.

Autor:	Saleh AA; Medical Biochemistry & Molecular Biology, Menoufia University, Egypt., Kasem HE; Internal Medicine Departments, Faculty of Medicine, Menoufia University, Egypt., Zahran ES; Internal Medicine Departments, Faculty of Medicine, Menoufia University, Egypt., El-Hefnawy SM; Medical Biochemistry & Molecular Biology, Menoufia University, Egypt.
Jazyk:	angličtina
Zdroj:	Biochemistry and biophysics reports [Biochem Biophys Rep] 2020 Jun 02; Vol. 23, pp. 100770. Date of Electronic Publication: 2020 Jun 02 (Print Publication: 2020).
DOI:	10.1016/j.bbrep.2020.100770
Abstrakt:	Background: Increasing interest has been focused on lncRNAs as potential markers in the pathogenesis and progression of numerous diseases. Aim: We aimed to investigate the expression pattern and role of cell-free lncRNAs (GAS5, HCG27_201 and LY86-AS1) in pre-diabetic, diabetic and T2DM groups. Subjects & Methods: Quantification of the expression level of cell-free lncRNAs (GAS5, HCG27_201 and LY86-AS1) was performed by real-time PCR in 210 individuals classified in diabetic (T2DM), pre-diabetic and control groups. Results: Significant differences were observed in the relative expression level of lncRNAs (GAS5, LY86-AS1 and HCG27_201) among the three studied groups. The LncRNA expression levels decreased gradually from the control to the pre-diabetic group and reached the lowest values in the T2DM group. The A receiver operating characteristic curve (ROC) was applied to identify a cut-off value for each of the three genes among our groups. The three lncRNAs showed promising results in discriminating between the diabetic patients and controls, with HCG27_201 gene expression having the best performance. Furthermore, lncRNA expression was able to predict the future development of DM in the pre-diabetics because ROC analysis among diabetics and pre-diabetics revealed considerable results. GAS5 gene expression showed the best performance. Additionally, HCG27_201 expression was the most valuable biomarker for differentiating between pre-diabetics and controls and presented a sensitivity of 91% and specificity of 64%. Conclusions: We concluded that cell free lncRNAs (GAS5, LY86-AS1 and HCG27_201) could be considered promising diagnostic and predictive biomarkers for DM and that HCG27_201 could act as a potential diagnostic biomarker for pre-diabetes. Competing Interests: There is no conflict of interest among authors. (© 2020 Published by Elsevier B.V.)
Databáze:	MEDLINE
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